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Human skeletal stem cell response to multiscale topography induced by large area electron beam melting surface treatment

Human skeletal stem cell response to multiscale topography induced by large area electron beam melting surface treatment
Human skeletal stem cell response to multiscale topography induced by large area electron beam melting surface treatment
The healthcare socio-economic environment is irreversibly changing as a consequence of an increasing aging population, consequent functional impairment, and patient quality of life expectations. The increasing complexity of ensuing clinical scenarios compels a critical search for novel musculoskeletal regenerative and replacement strategies. While joint arthroplasty is a highly effective treatment for arthritis and osteoporosis, further innovation and refinement of uncemented implants are essential in order to improve implant integration and reduce implant revision rate. This is critical given financial restraints and the drive to improve cost-effectiveness and quality of life outcomes. Multi-scale modulation of implant surfaces, offers an innovative approach to enhancement in implant performance. In the current study, we have examined the potential of large area electron beam melting to alter the surface nanotopography in titanium alloy (Ti6Al4V). We evaluated the in vitro osteogenic response of human skeletal stem cells to the resultant nanotopography, providing evidence of the relationship between the biological response, particularly Collagen type I and Osteocalcin gene activation, and surface nanoroughness. The current studies demonstrate osteogenic gene induction and morphological cell changes to be significantly enhanced on a topography Ra of ~40 nm with clinical implications therein for implant surface treatment and generation.
1-12
Oreffo, Richard
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Gorianov, Vitaly
e2d8b62b-fe72-45de-b469-309e6c9f3293
Cook, Richard
b14975ee-e08c-40a9-8dd0-f8ed2c1a799b
Walker, John
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Dunlop, D.G.
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Clare, Adam
bb20eb07-54cc-4758-8404-fb8e177317b8
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Gorianov, Vitaly
e2d8b62b-fe72-45de-b469-309e6c9f3293
Cook, Richard
b14975ee-e08c-40a9-8dd0-f8ed2c1a799b
Walker, John
6a9c9c5e-9b0e-4de1-9af9-63d7c866cf86
Dunlop, D.G.
eb03bdad-6a88-4057-acd8-70a9271f629d
Clare, Adam
bb20eb07-54cc-4758-8404-fb8e177317b8

Oreffo, Richard, Gorianov, Vitaly, Cook, Richard, Walker, John, Dunlop, D.G. and Clare, Adam (2018) Human skeletal stem cell response to multiscale topography induced by large area electron beam melting surface treatment. Frontiers in Bioengineering and Biotechnology, 1-12. (doi:10.3389/fbioe.2018.00091).

Record type: Article

Abstract

The healthcare socio-economic environment is irreversibly changing as a consequence of an increasing aging population, consequent functional impairment, and patient quality of life expectations. The increasing complexity of ensuing clinical scenarios compels a critical search for novel musculoskeletal regenerative and replacement strategies. While joint arthroplasty is a highly effective treatment for arthritis and osteoporosis, further innovation and refinement of uncemented implants are essential in order to improve implant integration and reduce implant revision rate. This is critical given financial restraints and the drive to improve cost-effectiveness and quality of life outcomes. Multi-scale modulation of implant surfaces, offers an innovative approach to enhancement in implant performance. In the current study, we have examined the potential of large area electron beam melting to alter the surface nanotopography in titanium alloy (Ti6Al4V). We evaluated the in vitro osteogenic response of human skeletal stem cells to the resultant nanotopography, providing evidence of the relationship between the biological response, particularly Collagen type I and Osteocalcin gene activation, and surface nanoroughness. The current studies demonstrate osteogenic gene induction and morphological cell changes to be significantly enhanced on a topography Ra of ~40 nm with clinical implications therein for implant surface treatment and generation.

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Accepted/In Press date: 19 June 2018
e-pub ahead of print date: 24 July 2018

Identifiers

Local EPrints ID: 422859
URI: http://eprints.soton.ac.uk/id/eprint/422859
PURE UUID: 114c09d6-e25a-41b9-a6a8-8e42b47c7fae
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 07 Aug 2018 16:30
Last modified: 07 Oct 2020 01:44

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