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Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in europe

Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in europe
Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in europe

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre-or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

Canker, Multi-locus sequence typing, Pathogenicity, Vitis
0031-5850
135-153
Guarnaccia, V.
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Groenewald, J. Z.
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Woodhall, J.
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Armengol, J.
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Cinelli, T.
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Eichmeier, A.
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Ezra, D.
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Fontaine, F.
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Gramaje, D.
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Gutierrez-Aguirregabiria, A.
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Kaliterna, J.
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Kiss, L.
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Larignon, P.
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Luque, J.
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Mugnai, L.
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Naor, V.
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Raposo, R.
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Sándor, E.
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Váczy, K. Z.
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Crous, P. W.
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Guarnaccia, V.
e089a418-0c27-4974-8067-8675312cc938
Groenewald, J. Z.
44747141-633b-42b4-bb33-28bfe1f8abbd
Woodhall, J.
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Armengol, J.
04339aa8-a228-4bba-9eff-686d3b3601bd
Cinelli, T.
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Eichmeier, A.
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Ezra, D.
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Fontaine, F.
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Gramaje, D.
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Gutierrez-Aguirregabiria, A.
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Kaliterna, J.
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Kiss, L.
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Larignon, P.
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Luque, J.
bee2b5e2-5041-4ea3-a1da-29a02b1d045c
Mugnai, L.
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Naor, V.
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Raposo, R.
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Sándor, E.
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Váczy, K. Z.
4fc6223d-1198-48b7-9c86-36506a400a9a
Crous, P. W.
57a39b6b-8f39-4254-8f0d-8bc86d703dd4

Guarnaccia, V., Groenewald, J. Z., Woodhall, J., Armengol, J., Cinelli, T., Eichmeier, A., Ezra, D., Fontaine, F., Gramaje, D., Gutierrez-Aguirregabiria, A., Kaliterna, J., Kiss, L., Larignon, P., Luque, J., Mugnai, L., Naor, V., Raposo, R., Sándor, E., Váczy, K. Z. and Crous, P. W. (2018) Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in europe. Persoonia: Molecular Phylogeny and Evolution of Fungi, 40, 135-153. (doi:10.3767/persoonia.2018.40.06).

Record type: Article

Abstract

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre-or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

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Accepted/In Press date: 5 January 2018
e-pub ahead of print date: 19 February 2018
Keywords: Canker, Multi-locus sequence typing, Pathogenicity, Vitis

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Local EPrints ID: 422998
URI: http://eprints.soton.ac.uk/id/eprint/422998
ISSN: 0031-5850
PURE UUID: 05e271dd-b0f5-4ddc-aaf6-cb7fa4a7baf3

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Date deposited: 09 Aug 2018 16:30
Last modified: 14 Sep 2021 18:51

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Contributors

Author: V. Guarnaccia
Author: J. Z. Groenewald
Author: J. Woodhall
Author: J. Armengol
Author: T. Cinelli
Author: A. Eichmeier
Author: D. Ezra
Author: F. Fontaine
Author: D. Gramaje
Author: A. Gutierrez-Aguirregabiria
Author: J. Kaliterna
Author: L. Kiss
Author: P. Larignon
Author: J. Luque
Author: L. Mugnai
Author: V. Naor
Author: R. Raposo
Author: E. Sándor
Author: K. Z. Váczy
Author: P. W. Crous

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