The University of Southampton
University of Southampton Institutional Repository

Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute

Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute
Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute

Purpose: To develop and optimise a co-polymer blend of poly(ethylene glycol methacrylate) (PEGM) and poly(methyl methacrylate) (PMMA) to act as a synthetic Bruch’s membrane (BrM) scaffold for primary murine retinal pigment epithelium (RPE) monolayers, as a potential treatment for dry age-related macular degeneration (AMD). This bio-compatible polymer film is designed to mimic BrM and to allow RPE cells to attach to the surface and proliferate, whilst retaining the necessary properties of human BrM, including porosity, thickness, biocompatibility and adhesion for the anchorage-dependant RPE cells.

Methods: PEGM and PMMA are biocompatible polymers which are already in use for clinical applications. This co-polymer blend is functionalised at the PEGM site with N,N’-Disuccinimidyl carbonate and a RGDS peptide to facilitate RPE attachment. Electrospinning is used to produce polymer fibre sheets. By altering the parameters of the electrospinning apparatus (voltage, distance from needle to collector, flow rate) we can control the polymer fibre production.

Results: By using the electrospinning technique, the co-polymer mat provides the necessary porosity, and by altering the parameters of the electrospinning, the thickness has been optimised to match human BrM (confirmed via an optical profiler, which found that the co-polymer had a thickness of approximately 12µm). The fidelity and effectiveness of the PMMA:PEGM co-polymer have been tested using long-term cultures of primary murine RPE monolayers. Our experiments also characterised RPE-barrier properties to establish the suitability of this novel synthetic scaffold for future transplantation studies

Conclusions: This work demonstrates synthesis of a PMMA:PEGM co-polymer film, through the use of electrospinning, which mimics the properties of the BrM (porosity, thickness, biocompatibility and adhesion). This suggests that the electrospun membrane is a viable scaffold to facilitate delivery of RPE cells to the sub-retinal space. This is an exciting prospect and future work will investigate sub-retinal delivery of the co-polymer, via use of an in house designed and manufactured injector.
0146-0404
2997
Ward, Gareth
250b5aa5-a94a-49ec-9569-9250d8e2607b
Thomson, Heather
8914c4e4-b602-4ecc-a99d-897ed0fdd472
Alexander, Philip
ea648f08-18ec-4834-befc-9d2279e7f2bc
Ratnayaka, J. Arjuna
002499b8-1a9f-45b6-9539-5ac145799dfd
Treharne, Andrew
a2c4ab5e-b323-4f3f-aa6f-ec3928f45075
Attard, George
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Grossel, Martin
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Ward, Gareth
250b5aa5-a94a-49ec-9569-9250d8e2607b
Thomson, Heather
8914c4e4-b602-4ecc-a99d-897ed0fdd472
Alexander, Philip
ea648f08-18ec-4834-befc-9d2279e7f2bc
Ratnayaka, J. Arjuna
002499b8-1a9f-45b6-9539-5ac145799dfd
Treharne, Andrew
a2c4ab5e-b323-4f3f-aa6f-ec3928f45075
Attard, George
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Grossel, Martin
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514

Ward, Gareth, Thomson, Heather, Alexander, Philip, Ratnayaka, J. Arjuna, Treharne, Andrew, Attard, George, Grossel, Martin and Lotery, Andrew (2014) Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute. Investigative Ophthalmology & Visual Science, 55 (13), 2997.

Record type: Meeting abstract

Abstract


Purpose: To develop and optimise a co-polymer blend of poly(ethylene glycol methacrylate) (PEGM) and poly(methyl methacrylate) (PMMA) to act as a synthetic Bruch’s membrane (BrM) scaffold for primary murine retinal pigment epithelium (RPE) monolayers, as a potential treatment for dry age-related macular degeneration (AMD). This bio-compatible polymer film is designed to mimic BrM and to allow RPE cells to attach to the surface and proliferate, whilst retaining the necessary properties of human BrM, including porosity, thickness, biocompatibility and adhesion for the anchorage-dependant RPE cells.

Methods: PEGM and PMMA are biocompatible polymers which are already in use for clinical applications. This co-polymer blend is functionalised at the PEGM site with N,N’-Disuccinimidyl carbonate and a RGDS peptide to facilitate RPE attachment. Electrospinning is used to produce polymer fibre sheets. By altering the parameters of the electrospinning apparatus (voltage, distance from needle to collector, flow rate) we can control the polymer fibre production.

Results: By using the electrospinning technique, the co-polymer mat provides the necessary porosity, and by altering the parameters of the electrospinning, the thickness has been optimised to match human BrM (confirmed via an optical profiler, which found that the co-polymer had a thickness of approximately 12µm). The fidelity and effectiveness of the PMMA:PEGM co-polymer have been tested using long-term cultures of primary murine RPE monolayers. Our experiments also characterised RPE-barrier properties to establish the suitability of this novel synthetic scaffold for future transplantation studies

Conclusions: This work demonstrates synthesis of a PMMA:PEGM co-polymer film, through the use of electrospinning, which mimics the properties of the BrM (porosity, thickness, biocompatibility and adhesion). This suggests that the electrospun membrane is a viable scaffold to facilitate delivery of RPE cells to the sub-retinal space. This is an exciting prospect and future work will investigate sub-retinal delivery of the co-polymer, via use of an in house designed and manufactured injector.

Full text not available from this repository.

More information

Published date: 1 April 2014

Identifiers

Local EPrints ID: 423097
URI: http://eprints.soton.ac.uk/id/eprint/423097
ISSN: 0146-0404
PURE UUID: e89e40c1-ac19-47c3-99be-ff3c859f60a3
ORCID for J. Arjuna Ratnayaka: ORCID iD orcid.org/0000-0002-1027-6938
ORCID for George Attard: ORCID iD orcid.org/0000-0001-8304-0742
ORCID for Martin Grossel: ORCID iD orcid.org/0000-0001-7469-6854
ORCID for Andrew Lotery: ORCID iD orcid.org/0000-0001-5541-4305

Catalogue record

Date deposited: 14 Aug 2018 16:30
Last modified: 18 Feb 2021 17:22

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×