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Dielectric characterization of Plasmodium falciparum infected red blood cells using microfluidic impedance cytometry

Dielectric characterization of Plasmodium falciparum infected red blood cells using microfluidic impedance cytometry
Dielectric characterization of Plasmodium falciparum infected red blood cells using microfluidic impedance cytometry
Although malaria is the world’s most life-threatening parasitic disease, there is no clear understanding of how certain biophysical properties of infected cells change during the malaria infection cycle. In this article, we use microfluidic impedance cytometry to measure the dielectric properties of Plasmodium falciparum-infected red blood cells (i-RBCs) at specific time-points during the infection cycle. Individual parasites were identified within i-RBCs using Green Fluorescent Protein (GFP) emission. The dielectric properties of cell sub-populations were determined using the multi-shell model. Analysis showed that the membrane capacitance and cytoplasmic conductivity of i-RBCs increased along the infection time-course, due to membrane alterations caused by parasite infection. The volume ratio occupied by the parasite was estimated to vary from <10% at earlier stages, to ~90% at later stages. This knowledge could be used to develop new label-free cell sorting techniques for sample pre-enrichment, improving diagnosis.
Human Malaria, Plasmodium falciparum, Impedance cytometry, Dielectric characterization, Microfluidics
1742-5689
Honrado, Carlos, Manuel Fernandes
5d2caaca-3cab-432b-94d0-6793e3514c99
Ciuffreda, Laura
1421923e-82f0-42f8-8794-e9ae3044c0ae
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Ranford-Cartwright, Lisa
656131ad-3dd6-4a4f-aa93-95fcb6f96285
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Honrado, Carlos, Manuel Fernandes
5d2caaca-3cab-432b-94d0-6793e3514c99
Ciuffreda, Laura
1421923e-82f0-42f8-8794-e9ae3044c0ae
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Ranford-Cartwright, Lisa
656131ad-3dd6-4a4f-aa93-95fcb6f96285
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Honrado, Carlos, Manuel Fernandes, Ciuffreda, Laura, Spencer, Daniel, Ranford-Cartwright, Lisa and Morgan, Hywel (2018) Dielectric characterization of Plasmodium falciparum infected red blood cells using microfluidic impedance cytometry. Journal of the Royal Society Interface, 15 (147). (doi:10.1098/rsif.2018.0416).

Record type: Article

Abstract

Although malaria is the world’s most life-threatening parasitic disease, there is no clear understanding of how certain biophysical properties of infected cells change during the malaria infection cycle. In this article, we use microfluidic impedance cytometry to measure the dielectric properties of Plasmodium falciparum-infected red blood cells (i-RBCs) at specific time-points during the infection cycle. Individual parasites were identified within i-RBCs using Green Fluorescent Protein (GFP) emission. The dielectric properties of cell sub-populations were determined using the multi-shell model. Analysis showed that the membrane capacitance and cytoplasmic conductivity of i-RBCs increased along the infection time-course, due to membrane alterations caused by parasite infection. The volume ratio occupied by the parasite was estimated to vary from <10% at earlier stages, to ~90% at later stages. This knowledge could be used to develop new label-free cell sorting techniques for sample pre-enrichment, improving diagnosis.

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Accepted/In Press date: 5 September 2018
e-pub ahead of print date: 17 October 2018
Keywords: Human Malaria, Plasmodium falciparum, Impedance cytometry, Dielectric characterization, Microfluidics

Identifiers

Local EPrints ID: 423193
URI: https://eprints.soton.ac.uk/id/eprint/423193
ISSN: 1742-5689
PURE UUID: 53273bd4-5846-4db3-96ed-450f666ef04c
ORCID for Carlos, Manuel Fernandes Honrado: ORCID iD orcid.org/0000-0002-4151-2147
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 19 Sep 2018 16:30
Last modified: 17 Sep 2019 00:52

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Contributors

Author: Carlos, Manuel Fernandes Honrado ORCID iD
Author: Laura Ciuffreda
Author: Daniel Spencer
Author: Lisa Ranford-Cartwright
Author: Hywel Morgan ORCID iD

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