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Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging

Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging
Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging
The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration. However, there remains a crucial unmet need to develop quantitative tools to elucidate skeletal cell development and monitor the formation of regenerated tissues using non-destructive techniques in 3D. Label-free methods such as coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon excited auto-fluorescence (TPEAF) microscopy are minimally invasive, non-destructive, and present new powerful alternatives to conventional imaging techniques. Here we report a combination of these techniques in a single multimodal system for the temporal assessment of cartilage formation by human skeletal cells. The evaluation of bioengineered cartilage, with a new parameter measuring the amount of collagen per cell, collagen fibre structure and chondrocyte distribution, was performed using the 3D non-destructive platform. Such 3D label-free temporal quantification paves the way for tracking skeletal cell development in real-time and offers a paradigm shift in tissue engineering and regenerative medicine applications.
Moura, Catarina Costa
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Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Montfort, Tual
c98812fb-75b4-43bd-9e34-93567b8fa64d
Bourdakos, Konstantinos N.
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Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Oreffo, Richard O.C.
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Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Moura, Catarina Costa
fdd85e38-f46f-47d0-a33a-6e7328398e90
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Montfort, Tual
c98812fb-75b4-43bd-9e34-93567b8fa64d
Bourdakos, Konstantinos N.
83f6fc3a-db12-476b-9a78-4aad8756f82f
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9

Moura, Catarina Costa, Lanham, Stuart A., Montfort, Tual, Bourdakos, Konstantinos N., Tare, Rahul S., Oreffo, Richard O.C. and Mahajan, Sumeet (2018) Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging. Integrative Biology. (doi:10.1039/C8IB00050F).

Record type: Article

Abstract

The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration. However, there remains a crucial unmet need to develop quantitative tools to elucidate skeletal cell development and monitor the formation of regenerated tissues using non-destructive techniques in 3D. Label-free methods such as coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon excited auto-fluorescence (TPEAF) microscopy are minimally invasive, non-destructive, and present new powerful alternatives to conventional imaging techniques. Here we report a combination of these techniques in a single multimodal system for the temporal assessment of cartilage formation by human skeletal cells. The evaluation of bioengineered cartilage, with a new parameter measuring the amount of collagen per cell, collagen fibre structure and chondrocyte distribution, was performed using the 3D non-destructive platform. Such 3D label-free temporal quantification paves the way for tracking skeletal cell development in real-time and offers a paradigm shift in tissue engineering and regenerative medicine applications.

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Accepted/In Press date: 5 September 2018
e-pub ahead of print date: 5 September 2018

Identifiers

Local EPrints ID: 423533
URI: http://eprints.soton.ac.uk/id/eprint/423533
PURE UUID: 8376c559-c08c-4997-a94b-97820b3936ea
ORCID for Stuart A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Rahul S. Tare: ORCID iD orcid.org/0000-0001-8274-8837
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

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Date deposited: 25 Sep 2018 16:30
Last modified: 18 Feb 2021 17:09

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