A novel resolution measure for optical microscopes: Stochastic analysis of the performance limits
A novel resolution measure for optical microscopes: Stochastic analysis of the performance limits
In the recent past, the increased use of optical microscopes in quantitative studies, such as single molecule microscopy, has generated significant interest in quantifying its performance limit. Here, by adopting an information-theoretic stochastic framework, we present expressions to calculate performance limits that quantify the capabilities of optical microscopes. We revisit the resolution problem from the stochastic framework and derive a new resolution measure. Our result, unlike Rayleigh's resolution criterion, predicts that the resolution of an optical microscope is not limited, but that the resolvability depends on the detected photon count. Analytical expressions are also given that take into account the effect of deteriorating experimental factors such as pixelation and noise sources. We also consider the location estimation problem, which is of relevance for particle tracking applications.
770-773
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
2006
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ram, Sripad, Ward, E. Sally and Ober, Raimund J.
(2006)
A novel resolution measure for optical microscopes: Stochastic analysis of the performance limits.
In 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings.
vol. 2006,
IEEE.
.
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Conference or Workshop Item
(Paper)
Abstract
In the recent past, the increased use of optical microscopes in quantitative studies, such as single molecule microscopy, has generated significant interest in quantifying its performance limit. Here, by adopting an information-theoretic stochastic framework, we present expressions to calculate performance limits that quantify the capabilities of optical microscopes. We revisit the resolution problem from the stochastic framework and derive a new resolution measure. Our result, unlike Rayleigh's resolution criterion, predicts that the resolution of an optical microscope is not limited, but that the resolvability depends on the detected photon count. Analytical expressions are also given that take into account the effect of deteriorating experimental factors such as pixelation and noise sources. We also consider the location estimation problem, which is of relevance for particle tracking applications.
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Published date: 2006
Venue - Dates:
2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro, , Arlington, VA, United States, 2006-04-06 - 2006-04-09
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Local EPrints ID: 423585
URI: http://eprints.soton.ac.uk/id/eprint/423585
PURE UUID: 2a387a6c-9a6d-47cd-ad7c-4d783b3b1554
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Date deposited: 27 Sep 2018 16:30
Last modified: 06 Jun 2024 02:04
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Author:
Sripad Ram
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