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3D resolution measure for multifocal plane microscopy

3D resolution measure for multifocal plane microscopy
3D resolution measure for multifocal plane microscopy

The distance separating two biomolecules in close proximity is an important determinant of the nature of their interaction. While much focus has been given to resolving distances in 2D, the 3D cell in which biological interactions occur necessitates the evaluation of resolution in 3D. Recently, we introduced an information-theoretic 3D resolution measure which predicts that the resolution of an optical microscope is unlimited, and that it improves as more photons are detected from the imaged molecules. Here, we investigate the 3D resolution measure for a multifocal plane microscope. Used for the simultaneous imaging of distinct focal planes within a specimen, multifocal plane microscopy has important applications in the tracking of microscopic objects in 3D. By comparing their 3D resolution measures, we determine the circumstances under which a two-plane microscope setup offers better resolvability than a comparable conventional microscope.

Cramer-Rao inequality, Fisher information matrix, Multifocal plane microscopy, Resolution measure
1339-1342
Institute of Electrical and Electronics Engineers Inc.
Chao, Jerry
550e20b0-8365-42e3-a6fc-1048eb8c2e47
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Chao, Jerry
550e20b0-8365-42e3-a6fc-1048eb8c2e47
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36

Chao, Jerry, Ram, Sripad, Ward, E. Sally and Ober, Raimund J. (2008) 3D resolution measure for multifocal plane microscopy. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. Institute of Electrical and Electronics Engineers Inc. pp. 1339-1342 . (doi:10.1109/ISBI.2008.4541252).

Record type: Conference or Workshop Item (Paper)

Abstract

The distance separating two biomolecules in close proximity is an important determinant of the nature of their interaction. While much focus has been given to resolving distances in 2D, the 3D cell in which biological interactions occur necessitates the evaluation of resolution in 3D. Recently, we introduced an information-theoretic 3D resolution measure which predicts that the resolution of an optical microscope is unlimited, and that it improves as more photons are detected from the imaged molecules. Here, we investigate the 3D resolution measure for a multifocal plane microscope. Used for the simultaneous imaging of distinct focal planes within a specimen, multifocal plane microscopy has important applications in the tracking of microscopic objects in 3D. By comparing their 3D resolution measures, we determine the circumstances under which a two-plane microscope setup offers better resolvability than a comparable conventional microscope.

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More information

e-pub ahead of print date: 13 June 2008
Published date: 2008
Venue - Dates: 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI, Paris, France, 2008-05-14 - 2008-05-17
Keywords: Cramer-Rao inequality, Fisher information matrix, Multifocal plane microscopy, Resolution measure

Identifiers

Local EPrints ID: 423599
URI: https://eprints.soton.ac.uk/id/eprint/423599
PURE UUID: cafd79b6-38ca-4fe6-9b02-da311aaf041f
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238
ORCID for Raimund J. Ober: ORCID iD orcid.org/0000-0002-1290-7430

Catalogue record

Date deposited: 27 Sep 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: Jerry Chao
Author: Sripad Ram
Author: E. Sally Ward ORCID iD
Author: Raimund J. Ober ORCID iD

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