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Fisher information for EMCCD imaging with application to single molecule microscopy

Fisher information for EMCCD imaging with application to single molecule microscopy
Fisher information for EMCCD imaging with application to single molecule microscopy

Owing to its high quantum efficiency, the charge-coupled device (CCD) is an important imaging tool employed in biological applications such as single molecule microscopy. Under extremely low light conditions, however, a CCD is generally unsuitable because its readout noise can easily overwhelm the weak signal. Instead, an electron-multiplying charge-coupled device (EMCCD), which stochastically amplifies the acquired signal to drown out the readout noise, can be used. We have previously proposed a framework for calculating the Fisher information, and hence the Cramer-Rao lower bound, for estimating parameters (e.g., single molecule location) from the images produced by an optical microscope. Here, we develop the theory that is needed for deriving, within this framework, performance measures pertaining to the estimation of parameters from an EMCCD image. Our results allow the comparison of a CCD and an EMCCD in terms of the best accuracy with which parameters can be estimated from their acquired images.

Branching process, electron multiplication, Fisher information, single molecule microscopy
1085-1089
Institute of Electrical and Electronics Engineers Inc.
Chao, Jerry
550e20b0-8365-42e3-a6fc-1048eb8c2e47
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Chao, Jerry
550e20b0-8365-42e3-a6fc-1048eb8c2e47
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36

Chao, Jerry, Ward, E. Sally and Ober, Raimund J. (2010) Fisher information for EMCCD imaging with application to single molecule microscopy. In Conference Record of the 44th Asilomar Conference on Signals, Systems and Computers, Asilomar 2010. Institute of Electrical and Electronics Engineers Inc. pp. 1085-1089 . (doi:10.1109/ACSSC.2010.5757570).

Record type: Conference or Workshop Item (Paper)

Abstract

Owing to its high quantum efficiency, the charge-coupled device (CCD) is an important imaging tool employed in biological applications such as single molecule microscopy. Under extremely low light conditions, however, a CCD is generally unsuitable because its readout noise can easily overwhelm the weak signal. Instead, an electron-multiplying charge-coupled device (EMCCD), which stochastically amplifies the acquired signal to drown out the readout noise, can be used. We have previously proposed a framework for calculating the Fisher information, and hence the Cramer-Rao lower bound, for estimating parameters (e.g., single molecule location) from the images produced by an optical microscope. Here, we develop the theory that is needed for deriving, within this framework, performance measures pertaining to the estimation of parameters from an EMCCD image. Our results allow the comparison of a CCD and an EMCCD in terms of the best accuracy with which parameters can be estimated from their acquired images.

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More information

Published date: 2010
Venue - Dates: 44th Asilomar Conference on Signals, Systems and Computers, Asilomar 2010, Pacific Grove, CA, United States, 2010-11-07 - 2010-11-10
Keywords: Branching process, electron multiplication, Fisher information, single molecule microscopy

Identifiers

Local EPrints ID: 423625
URI: http://eprints.soton.ac.uk/id/eprint/423625
PURE UUID: ae0cac89-533c-403a-8815-a9daa0a5e134
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238
ORCID for Raimund J. Ober: ORCID iD orcid.org/0000-0002-1290-7430

Catalogue record

Date deposited: 27 Sep 2018 16:30
Last modified: 20 Jul 2019 00:22

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Contributors

Author: Jerry Chao
Author: E. Sally Ward ORCID iD
Author: Raimund J. Ober ORCID iD

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