3D single molecule tracking and superresolution microscopy using multifocal plane microscopy
3D single molecule tracking and superresolution microscopy using multifocal plane microscopy
The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.
2D/3D localization accuracy, Cramer-Rao lower bound, Fisher information matrix, maximum likelihood estimator, resolution limits
914-915
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
2012
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ram, Sripad, Ward, E. Sally and Ober, Raimund J.
(2012)
3D single molecule tracking and superresolution microscopy using multifocal plane microscopy.
In 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings.
IEEE.
.
(doi:10.1109/ISBI.2012.6235702).
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Conference or Workshop Item
(Paper)
Abstract
The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.
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Published date: 2012
Venue - Dates:
2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macr, , Barcelona, Spain, 2012-05-02 - 2012-05-05
Keywords:
2D/3D localization accuracy, Cramer-Rao lower bound, Fisher information matrix, maximum likelihood estimator, resolution limits
Identifiers
Local EPrints ID: 423631
URI: http://eprints.soton.ac.uk/id/eprint/423631
PURE UUID: a3b8626f-e4bd-4b11-a8ef-1fc5937ba6a7
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Date deposited: 27 Sep 2018 16:30
Last modified: 16 Mar 2024 04:37
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Author:
Sripad Ram
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