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Measurement errors in fluorescence microscopy image registration

Measurement errors in fluorescence microscopy image registration
Measurement errors in fluorescence microscopy image registration

Image registration is an important processing step in fluorescence microscopy, for example in tracking or super-resolution methods. Precision localization of single fluorescent molecules from a quantum limited photon detection process, subject to Gaussian readout noise, is key to the use of single molecule microscopy. It is therefore important to know the effect that registration has on the accuracy of localizing a single molecule. Here we demonstrate a suitable approach to image registration that accounts for point-wise errors in localizing the control points typically used in fluorescence microscopy. This allows expressions for the localization errors caused by the registration process to be derived, showing dependence on the number of control points and their associated photon counts.

1602-1606
Institute of Electrical and Electronics Engineers Inc.
Cohen, E. A.K.
97c75d1e-6c18-4e03-8e7a-29472b32d9c2
Ober, R. J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Cohen, E. A.K.
97c75d1e-6c18-4e03-8e7a-29472b32d9c2
Ober, R. J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36

Cohen, E. A.K. and Ober, R. J. (2012) Measurement errors in fluorescence microscopy image registration. In Conference Record of the 46th Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2012. Institute of Electrical and Electronics Engineers Inc. pp. 1602-1606 . (doi:10.1109/ACSSC.2012.6489300).

Record type: Conference or Workshop Item (Paper)

Abstract

Image registration is an important processing step in fluorescence microscopy, for example in tracking or super-resolution methods. Precision localization of single fluorescent molecules from a quantum limited photon detection process, subject to Gaussian readout noise, is key to the use of single molecule microscopy. It is therefore important to know the effect that registration has on the accuracy of localizing a single molecule. Here we demonstrate a suitable approach to image registration that accounts for point-wise errors in localizing the control points typically used in fluorescence microscopy. This allows expressions for the localization errors caused by the registration process to be derived, showing dependence on the number of control points and their associated photon counts.

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More information

Published date: 2012
Venue - Dates: 46th Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2012, Pacific Grove, CA, United States, 2012-11-04 - 2012-11-07

Identifiers

Local EPrints ID: 423638
URI: https://eprints.soton.ac.uk/id/eprint/423638
PURE UUID: a2cb2676-4806-45a4-ba39-394dfcafc50f
ORCID for R. J. Ober: ORCID iD orcid.org/0000-0002-1290-7430

Catalogue record

Date deposited: 27 Sep 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: E. A.K. Cohen
Author: R. J. Ober ORCID iD

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