Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway
Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway
A major outstanding issue in cell biology is the lack of understanding of the contribution of tubulovesicular transport carriers (TCs) to intracellular trafficking pathways within 3D cellular environments. This is primarily due to the challenges associated with the use of microscopy techniques to track these highly motile, small compartments. In the present study we have used multifocal plane microscopy with localized photoactivation to overcome these limitations. Using this approach, we have characterized individual components constituting the recycling pathway of the receptor FcRn. Specifically, several different pathways followed by TCs that intersect with larger, relatively static sorting endosomes have been defined. These pathways include a novel 'looping' process in which TCs leave and return to the same sorting endosome. Significantly, TCs with different itineraries can be identified by associations with distinct complements of Rab GTPases, APPL1 and SNX4. These studies provide a framework for further analyses of the recycling pathway.
FcRn, Localized photoactivation, Multifocal plane microscopy, Receptor recycling, Transport carrier
1176-1188
Gan, Zhuo
cc085ff5-6cca-4337-a4ae-7be99c5e6032
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
1 March 2013
Gan, Zhuo
cc085ff5-6cca-4337-a4ae-7be99c5e6032
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Gan, Zhuo, Ram, Sripad, Ober, Raimund J. and Ward, E. Sally
(2013)
Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway.
Journal of Cell Science, 126 (5), .
(doi:10.1242/jcs.116327).
Abstract
A major outstanding issue in cell biology is the lack of understanding of the contribution of tubulovesicular transport carriers (TCs) to intracellular trafficking pathways within 3D cellular environments. This is primarily due to the challenges associated with the use of microscopy techniques to track these highly motile, small compartments. In the present study we have used multifocal plane microscopy with localized photoactivation to overcome these limitations. Using this approach, we have characterized individual components constituting the recycling pathway of the receptor FcRn. Specifically, several different pathways followed by TCs that intersect with larger, relatively static sorting endosomes have been defined. These pathways include a novel 'looping' process in which TCs leave and return to the same sorting endosome. Significantly, TCs with different itineraries can be identified by associations with distinct complements of Rab GTPases, APPL1 and SNX4. These studies provide a framework for further analyses of the recycling pathway.
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Accepted/In Press date: 10 December 2012
e-pub ahead of print date: 23 January 2013
Published date: 1 March 2013
Keywords:
FcRn, Localized photoactivation, Multifocal plane microscopy, Receptor recycling, Transport carrier
Identifiers
Local EPrints ID: 423647
URI: http://eprints.soton.ac.uk/id/eprint/423647
ISSN: 0021-9533
PURE UUID: f869d39f-cf8e-4124-a3e1-83cde166e4c1
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Date deposited: 27 Sep 2018 16:30
Last modified: 16 Mar 2024 04:37
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Author:
Zhuo Gan
Author:
Sripad Ram
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