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Efficacy of a novel ultrasonically activated water stream for removal of antimicrobial resistant biofilms

Efficacy of a novel ultrasonically activated water stream for removal of antimicrobial resistant biofilms
Efficacy of a novel ultrasonically activated water stream for removal of antimicrobial resistant biofilms
Aims & Objectives Bacterial biofilms are a significant problem in the management of chronic wounds, such as venous leg ulcers. In this phenotype, bacteria are protected from both antibiotics and physical removal. This study aimed to test the effectiveness of a novel ultrasonically activated fluid stream (UAS) in removing biofilm from biological, soft surfaces in an in vitro wound model. Two in vitro wound models were used: pig trotters; and a pre-wounded human skin model (EpiDerm™ FT, Mattek Inc, USA). Early stage biofilms were cultured within the wounds using fluorescent-tagged Pseudomonas aeruginosa pMF230 and SYTO-9 pre-stained E-MRSA-16. Wound models were cleaned with either 0.9% saline or the UAS at a flow rate of 2 L/min, and residual bacteria visualised by direct in situ epifluorescence microscopy.
Leighton, Timothy
3e5262ce-1d7d-42eb-b013-fcc5c286bbae
Leighton, Timothy
3e5262ce-1d7d-42eb-b013-fcc5c286bbae

Leighton, Timothy (2018) Efficacy of a novel ultrasonically activated water stream for removal of antimicrobial resistant biofilms. 5 pp .

Record type: Conference or Workshop Item (Poster)

Abstract

Aims & Objectives Bacterial biofilms are a significant problem in the management of chronic wounds, such as venous leg ulcers. In this phenotype, bacteria are protected from both antibiotics and physical removal. This study aimed to test the effectiveness of a novel ultrasonically activated fluid stream (UAS) in removing biofilm from biological, soft surfaces in an in vitro wound model. Two in vitro wound models were used: pig trotters; and a pre-wounded human skin model (EpiDerm™ FT, Mattek Inc, USA). Early stage biofilms were cultured within the wounds using fluorescent-tagged Pseudomonas aeruginosa pMF230 and SYTO-9 pre-stained E-MRSA-16. Wound models were cleaned with either 0.9% saline or the UAS at a flow rate of 2 L/min, and residual bacteria visualised by direct in situ epifluorescence microscopy.

Text
2018 - Voegeli et al Krakow wound conference poster - Version of Record
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Published date: 9 May 2018

Identifiers

Local EPrints ID: 423686
URI: http://eprints.soton.ac.uk/id/eprint/423686
PURE UUID: 81b66846-2fe7-4758-aade-5c2049560ce7
ORCID for Timothy Leighton: ORCID iD orcid.org/0000-0002-1649-8750

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Date deposited: 27 Sep 2018 16:30
Last modified: 30 Jun 2020 04:01

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