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Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers

Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers
Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers

Background: Lung epithelial lining fluid (ELF)—sampled through sputum induction—is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood. Objectives: To characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort. Methods: Induced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was used to group subjects with comparable sputum lipidomes in order to identify distinct ELF phenotypes. Results: The induced sputum lipidome was diverse, comprising a range of different molecular classes, including at least 75 glycerophospholipids, 13 sphingolipids, 5 sterol lipids and 12 neutral glycerolipids. TDA identified two distinct phenotypes differentiated by a higher total lipid content and specific enrichments of diacyl-glycerophosphocholines, -inositols and -glycerols in one group, with enrichments of sterols, glycolipids and sphingolipids in the other. Subjects presenting the lipid-rich ELF phenotype also had significantly higher BMI, but did not differ in respect of other demographic characteristics such as age or gender. Conclusions: We provide the first evidence that the ELF lipidome varies significantly between healthy individuals and propose that such differences are related to weight status, highlighting the potential impact of (over)nutrition on lung lipid metabolism.

Epithelial lining fluid, Induced sputum, Lipid metabolism, Lipidomics, Mass spectrometry, Pulmonary surfactant, Weight status
1573-3882
Brandsma, J.
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Goss, V.
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Yang, Xian
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Bakke, Per S.
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Caruso, Massimo
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Chanez, Pascal
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Dahlén, S. E.
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Fowler, S. J.
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Horvath, Ildiko
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Krug, Norbert
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Montuschi, Paolo
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Sanak, Marek
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Sandström, Thomas
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Shaw, Dominick E.
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Chung, Kian Fan
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Singer, Florian
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Fleming, L. J.
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Sousa, A. R.
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Pandis, Ioannis
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Bansal, A. T.
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Sterk, P. J.
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Djukanović, R.
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Postle, A.
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The U-BIOPRED Study Group
Brandsma, J.
b4c553dc-9444-466a-b352-25fd8fa25ee7
Goss, V.
ef02be5d-9318-4f7d-b076-3153555980d0
Yang, Xian
4558883a-3219-41b8-b128-db265b69fd3f
Bakke, Per S.
14e4943c-16b0-4f9f-ab31-a72c6f02b581
Caruso, Massimo
d6a59da8-4f93-4926-93a6-e7c3b90b480f
Chanez, Pascal
695ba8e7-e6b7-49db-a88f-e5f5a366b869
Dahlén, S. E.
9e18ae23-c6a1-413c-93fe-203949a4dcda
Fowler, S. J.
7a077c2e-22e7-4a0c-b781-eec92fb931c8
Horvath, Ildiko
57682232-afd3-47ac-8725-ba6e92dc06ae
Krug, Norbert
eee1c70e-5c60-4344-ac06-3e689491a771
Montuschi, Paolo
10568e50-5a5f-47b3-9ac8-6a630c30882c
Sanak, Marek
2d614d0e-797e-4941-869a-9987521fb92c
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Shaw, Dominick E.
83e98e0a-fd2d-4c44-9249-6ac3ebd9614f
Chung, Kian Fan
33cf2f88-c721-4573-8d6d-ea8da6b0498f
Singer, Florian
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Fleming, L. J.
9c75632b-dc6d-44c4-acea-ae13d116bd44
Sousa, A. R.
66270f3e-43c9-4d6a-a82f-ae83f5b02944
Pandis, Ioannis
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Bansal, A. T.
35fbf858-fffc-474a-b176-28ed5a20b9c8
Sterk, P. J.
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Djukanović, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Postle, A.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66

Brandsma, J., Goss, V., Yang, Xian, Bakke, Per S., Caruso, Massimo, Chanez, Pascal, Dahlén, S. E., Fowler, S. J., Horvath, Ildiko, Krug, Norbert, Montuschi, Paolo, Sanak, Marek, Sandström, Thomas, Shaw, Dominick E., Chung, Kian Fan, Singer, Florian, Fleming, L. J., Sousa, A. R., Pandis, Ioannis, Bansal, A. T., Sterk, P. J., Djukanović, R. and Postle, A. , The U-BIOPRED Study Group (2018) Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers. Metabolomics, 14 (10), [123]. (doi:10.1007/s11306-018-1412-2).

Record type: Article

Abstract

Background: Lung epithelial lining fluid (ELF)—sampled through sputum induction—is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood. Objectives: To characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort. Methods: Induced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was used to group subjects with comparable sputum lipidomes in order to identify distinct ELF phenotypes. Results: The induced sputum lipidome was diverse, comprising a range of different molecular classes, including at least 75 glycerophospholipids, 13 sphingolipids, 5 sterol lipids and 12 neutral glycerolipids. TDA identified two distinct phenotypes differentiated by a higher total lipid content and specific enrichments of diacyl-glycerophosphocholines, -inositols and -glycerols in one group, with enrichments of sterols, glycolipids and sphingolipids in the other. Subjects presenting the lipid-rich ELF phenotype also had significantly higher BMI, but did not differ in respect of other demographic characteristics such as age or gender. Conclusions: We provide the first evidence that the ELF lipidome varies significantly between healthy individuals and propose that such differences are related to weight status, highlighting the potential impact of (over)nutrition on lung lipid metabolism.

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Accepted/In Press date: 12 August 2018
e-pub ahead of print date: 17 September 2018
Published date: 1 October 2018
Keywords: Epithelial lining fluid, Induced sputum, Lipid metabolism, Lipidomics, Mass spectrometry, Pulmonary surfactant, Weight status

Identifiers

Local EPrints ID: 423755
URI: http://eprints.soton.ac.uk/id/eprint/423755
ISSN: 1573-3882
PURE UUID: 090200b1-390f-48f5-9aaf-87804872fc28
ORCID for R. Djukanović: ORCID iD orcid.org/0000-0001-6039-5612
ORCID for A. Postle: ORCID iD orcid.org/0000-0001-7361-0756

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Date deposited: 01 Oct 2018 16:30
Last modified: 15 Aug 2019 00:58

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Contributors

Author: J. Brandsma
Author: V. Goss
Author: Xian Yang
Author: Per S. Bakke
Author: Massimo Caruso
Author: Pascal Chanez
Author: S. E. Dahlén
Author: S. J. Fowler
Author: Ildiko Horvath
Author: Norbert Krug
Author: Paolo Montuschi
Author: Marek Sanak
Author: Thomas Sandström
Author: Dominick E. Shaw
Author: Kian Fan Chung
Author: Florian Singer
Author: L. J. Fleming
Author: A. R. Sousa
Author: Ioannis Pandis
Author: A. T. Bansal
Author: P. J. Sterk
Author: R. Djukanović ORCID iD
Author: A. Postle ORCID iD

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