Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn
Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn
The MHC class I-related receptor, FcRn, plays a central role in regulating the serum levels of IgG. FcRn is expressed in endothelial cells, suggesting that these cells may be involved in maintaining IgG levels. We have used live cell imaging of FcRn-green fluorescent protein transfected human endothelial cells to analyze the intracellular events that control IgG homeostasis. We show that segregation of FcRn-IgG complexes from unbound IgG occurs in the sorting endosome. FcRn or FcRn-IgG complexes are gradually depleted from sorting endosomes to ultimately generate multivesicular bodies whose contents are destined for lysosomal degradation. In addition, the pathways taken by FcRn and the transferrin receptor overlap, despite distinct mechanisms of ligand uptake. The studies provide a dynamic view of the trafficking of FcRn and its ligand and have relevance to understanding how FcRn functions to maintain IgG homeostasis.
2021-2029
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Martinez, Cruz
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Vaccaro, Carlos
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Zhou, Jinchun
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Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
15 February 2004
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Martinez, Cruz
cbab0de7-7dc0-4444-959e-37e41588563f
Vaccaro, Carlos
f576072e-379e-4f42-9bd7-595b2f5b7d58
Zhou, Jinchun
373ef03a-4fb7-4022-98c2-7332251c5c30
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J., Martinez, Cruz, Vaccaro, Carlos, Zhou, Jinchun and Ward, E. Sally
(2004)
Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn.
Journal of Immunology, 172 (4), .
(doi:10.4049/jimmunol.172.4.2021).
Abstract
The MHC class I-related receptor, FcRn, plays a central role in regulating the serum levels of IgG. FcRn is expressed in endothelial cells, suggesting that these cells may be involved in maintaining IgG levels. We have used live cell imaging of FcRn-green fluorescent protein transfected human endothelial cells to analyze the intracellular events that control IgG homeostasis. We show that segregation of FcRn-IgG complexes from unbound IgG occurs in the sorting endosome. FcRn or FcRn-IgG complexes are gradually depleted from sorting endosomes to ultimately generate multivesicular bodies whose contents are destined for lysosomal degradation. In addition, the pathways taken by FcRn and the transferrin receptor overlap, despite distinct mechanisms of ligand uptake. The studies provide a dynamic view of the trafficking of FcRn and its ligand and have relevance to understanding how FcRn functions to maintain IgG homeostasis.
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Accepted/In Press date: 24 November 2003
e-pub ahead of print date: 5 February 2004
Published date: 15 February 2004
Identifiers
Local EPrints ID: 424084
URI: http://eprints.soton.ac.uk/id/eprint/424084
ISSN: 0022-1767
PURE UUID: 1c255848-ed98-4ff1-b853-a9c1b37aaed0
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Date deposited: 04 Oct 2018 16:30
Last modified: 16 Mar 2024 04:37
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Contributors
Author:
Cruz Martinez
Author:
Carlos Vaccaro
Author:
Jinchun Zhou
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