Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions
Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions
The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.
Biological cells, Fluorescence microscopy, Three-dimensional (3-d) tracking, Trafficking pathway
237-242
Prabhat, Prashant
e79cffdb-4de8-42cc-b0f7-6d28f6d3c82e
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Sally Ward, E.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
December 2004
Prabhat, Prashant
e79cffdb-4de8-42cc-b0f7-6d28f6d3c82e
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Sally Ward, E.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Prabhat, Prashant, Ram, Sripad, Sally Ward, E. and Ober, Raimund J.
(2004)
Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions.
IEEE Transactions on Nanobioscience, 3 (4), .
(doi:10.1109/TNB.2004.837899).
Abstract
The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.
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e-pub ahead of print date: 6 December 2004
Published date: December 2004
Keywords:
Biological cells, Fluorescence microscopy, Three-dimensional (3-d) tracking, Trafficking pathway
Identifiers
Local EPrints ID: 424085
URI: http://eprints.soton.ac.uk/id/eprint/424085
ISSN: 1536-1241
PURE UUID: 783e6fe8-4990-4cd9-aa90-27d557663652
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Date deposited: 04 Oct 2018 16:30
Last modified: 06 Jun 2024 02:04
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Contributors
Author:
Prashant Prabhat
Author:
Sripad Ram
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