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Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions

Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions
Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions

The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.

Biological cells, Fluorescence microscopy, Three-dimensional (3-d) tracking, Trafficking pathway
1536-1241
237-242
Prabhat, Prashant
e79cffdb-4de8-42cc-b0f7-6d28f6d3c82e
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Sally Ward, E.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Prabhat, Prashant
e79cffdb-4de8-42cc-b0f7-6d28f6d3c82e
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Sally Ward, E.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36

Prabhat, Prashant, Ram, Sripad, Sally Ward, E. and Ober, Raimund J. (2004) Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions. IEEE Transactions on Nanobioscience, 3 (4), 237-242. (doi:10.1109/TNB.2004.837899).

Record type: Article

Abstract

The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.

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More information

e-pub ahead of print date: 6 December 2004
Published date: December 2004
Keywords: Biological cells, Fluorescence microscopy, Three-dimensional (3-d) tracking, Trafficking pathway

Identifiers

Local EPrints ID: 424085
URI: http://eprints.soton.ac.uk/id/eprint/424085
ISSN: 1536-1241
PURE UUID: 783e6fe8-4990-4cd9-aa90-27d557663652
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238
ORCID for Raimund J. Ober: ORCID iD orcid.org/0000-0002-1290-7430

Catalogue record

Date deposited: 04 Oct 2018 16:30
Last modified: 18 Mar 2024 03:48

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Contributors

Author: Prashant Prabhat
Author: Sripad Ram
Author: E. Sally Ward ORCID iD
Author: Raimund J. Ober ORCID iD

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