How accurately can a single molecule be localized when imaged through an optical microscope?
How accurately can a single molecule be localized when imaged through an optical microscope?
We present a simple analytical expression for the fundamental limit to the accuracy with which the location of a single molecule can be determined that is imaged through an optical microscope. This expression depends on the optical properties of the microscope and the photophysical properties of the single molecule. We also show how the fundamental limit is deteriorated by factors like pixelation of the detector and noise sources in the detection system. The present results gives an experimenter insight into what is achievable in an optical microscope and provide guidelines for experimental design.
1087-1090
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
2004
Ram, Sripad
559bd560-3817-4e53-8c7a-2f08e4518412
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ram, Sripad, Ward, E. Sally and Ober, Raimund J.
(2004)
How accurately can a single molecule be localized when imaged through an optical microscope?
In 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano.
vol. 2,
IEEE.
.
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Conference or Workshop Item
(Paper)
Abstract
We present a simple analytical expression for the fundamental limit to the accuracy with which the location of a single molecule can be determined that is imaged through an optical microscope. This expression depends on the optical properties of the microscope and the photophysical properties of the single molecule. We also show how the fundamental limit is deteriorated by factors like pixelation of the detector and noise sources in the detection system. The present results gives an experimenter insight into what is achievable in an optical microscope and provide guidelines for experimental design.
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Published date: 2004
Venue - Dates:
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, , Arlington, VA, United States, 2004-04-15 - 2004-04-18
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Local EPrints ID: 424086
URI: http://eprints.soton.ac.uk/id/eprint/424086
PURE UUID: 2de8772d-0470-40c1-b7ad-183e2686650b
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Date deposited: 04 Oct 2018 16:30
Last modified: 06 Mar 2024 03:00
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Author:
Sripad Ram
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