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Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study

Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study
Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study
Objective: subjects with low vitamin D levels are at risk of cardiometabolic disease. The aim of this study was to identify novel serological markers linking vitamin D status with cardiometabolic profile in non-diabetic adults with obesity.

Methods: for the discovery phase, we used quantitative serum proteomics in sex-matched, age-matched and BMI-matched subjects with obesity [BMI: 25–35 kg/m2] and low [25(OH)D < 50 nmol/L] vs. high vitamin D status [25(OH)D > 50 nmol/L] (n = 16). For the validation phase, we performed ELISA in a larger cohort with similar characteristics (n = 179).

Results: we identified 423 and 549 differentially expressed proteins in the high vs. low vitamin D groups of the male and female cohorts, respectively. The small molecule biochemistry protein networks and the glycolysis|gluconeogenesis pathway were significantly enriched in the DEPs of both sexes. As surrogate markers to these processes, the insulin-like growth factor binding protein -2 (IGFBP-2) was upregulated in males, whereas IGFBP-3 was upregulated in females from the high Vitamin D status. This sex-specific trend was confirmed using Luminex ELISA to an independent but clinically analogous cohort of males (n = 84, p = 0.002) and females (n = 95, p = 0.03).

Conclusions: the high Vitamin D status correlated with the serological upregulation of IGFBP-2 in males and IGFBP-3 in females with obesity and may constitute surrogate markers of risk reduction of cardiometabolic disease.
2044-4052
Al-Daghri, N.M.
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Manousopoulou, A.
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Alokail, M.S.
9adf2d61-d9f7-4095-bc36-701f0680646a
Yakout, S.
59a1ec84-40df-429c-8fc1-53febf12d89b
Alenad, A.
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Garay-Baquero, D.J.
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Fotopoulos, M.
05714262-55c5-48a7-af57-397d5dd1cd6a
Teng, J.
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Al-Attas, O.
4cb7dec9-090e-4718-9aab-36eae5c9514f
Al-Saleh, Y.
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Sabico, S.
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Chrousos, G.P.
f44fc9ca-5043-4256-bf27-0af4620b8001
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Al-Daghri, N.M.
4b252a9e-bd24-457f-a8c1-166d26e0239c
Manousopoulou, A.
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Alokail, M.S.
9adf2d61-d9f7-4095-bc36-701f0680646a
Yakout, S.
59a1ec84-40df-429c-8fc1-53febf12d89b
Alenad, A.
9b01a9ff-c314-4948-a705-4eb25d43d410
Garay-Baquero, D.J.
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Fotopoulos, M.
05714262-55c5-48a7-af57-397d5dd1cd6a
Teng, J.
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Al-Attas, O.
4cb7dec9-090e-4718-9aab-36eae5c9514f
Al-Saleh, Y.
b6a30c8e-441b-418f-9c77-cf61799fd2b1
Sabico, S.
8672f10e-65d7-412d-b616-5e6e625c9be5
Chrousos, G.P.
f44fc9ca-5043-4256-bf27-0af4620b8001
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd

Al-Daghri, N.M., Manousopoulou, A., Alokail, M.S., Yakout, S., Alenad, A., Garay-Baquero, D.J., Fotopoulos, M., Teng, J., Al-Attas, O., Al-Saleh, Y., Sabico, S., Chrousos, G.P. and Garbis, S.D. (2018) Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study. Nutrition & Diabetes, 8, [54]. (doi:10.1038/s41387-018-0063-8).

Record type: Article

Abstract

Objective: subjects with low vitamin D levels are at risk of cardiometabolic disease. The aim of this study was to identify novel serological markers linking vitamin D status with cardiometabolic profile in non-diabetic adults with obesity.

Methods: for the discovery phase, we used quantitative serum proteomics in sex-matched, age-matched and BMI-matched subjects with obesity [BMI: 25–35 kg/m2] and low [25(OH)D < 50 nmol/L] vs. high vitamin D status [25(OH)D > 50 nmol/L] (n = 16). For the validation phase, we performed ELISA in a larger cohort with similar characteristics (n = 179).

Results: we identified 423 and 549 differentially expressed proteins in the high vs. low vitamin D groups of the male and female cohorts, respectively. The small molecule biochemistry protein networks and the glycolysis|gluconeogenesis pathway were significantly enriched in the DEPs of both sexes. As surrogate markers to these processes, the insulin-like growth factor binding protein -2 (IGFBP-2) was upregulated in males, whereas IGFBP-3 was upregulated in females from the high Vitamin D status. This sex-specific trend was confirmed using Luminex ELISA to an independent but clinically analogous cohort of males (n = 84, p = 0.002) and females (n = 95, p = 0.03).

Conclusions: the high Vitamin D status correlated with the serological upregulation of IGFBP-2 in males and IGFBP-3 in females with obesity and may constitute surrogate markers of risk reduction of cardiometabolic disease.

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Sex-specific correlation of IGFBP-2 and -3 with vitamin D status in adults with obesity A cross-sectional serum proteomics study - Accepted Manuscript
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Al-Daghri et al 2018 Nutrition Diabetes - Version of Record
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More information

Accepted/In Press date: 10 September 2018
e-pub ahead of print date: 4 October 2018
Published date: 4 October 2018

Identifiers

Local EPrints ID: 424117
URI: http://eprints.soton.ac.uk/id/eprint/424117
ISSN: 2044-4052
PURE UUID: 89625d8a-58a0-4495-8384-32b261fc5241
ORCID for D.J. Garay-Baquero: ORCID iD orcid.org/0000-0002-9450-8504
ORCID for S.D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

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Date deposited: 04 Oct 2018 16:30
Last modified: 11 Jul 2024 02:03

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Contributors

Author: N.M. Al-Daghri
Author: A. Manousopoulou
Author: M.S. Alokail
Author: S. Yakout
Author: A. Alenad
Author: D.J. Garay-Baquero ORCID iD
Author: M. Fotopoulos
Author: J. Teng
Author: O. Al-Attas
Author: Y. Al-Saleh
Author: S. Sabico
Author: G.P. Chrousos
Author: S.D. Garbis ORCID iD

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