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Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study.

Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study.
Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study.
Objective

Subjects with low vitamin D levels are at risk of cardiometabolic disease. The aim of this study was to identify novel serological markers linking vitamin D status with cardiometabolic profile in non-diabetic adults with obesity.

Methods

For the discovery phase, we used quantitative serum proteomics in sex-matched, age-matched and BMI-matched subjects with obesity [BMI: 25–35 kg/m2] and low [25(OH)D < 50 nmol/L] vs. high vitamin D status [25(OH)D > 50 nmol/L] (n = 16). For the validation phase, we performed ELISA in a larger cohort with similar characteristics (n = 179).

Results

We identified 423 and 549 differentially expressed proteins in the high vs. low vitamin D groups of the male and female cohorts, respectively. The small molecule biochemistry protein networks and the glycolysis|gluconeogenesis pathway were significantly enriched in the DEPs of both sexes. As surrogate markers to these processes, the insulin-like growth factor binding protein -2  (IGFBP-2) was upregulated in males, whereas   IGFBP-3 was upregulated in females from the high Vitamin D status. This sex-specific trend  was confirmed using Luminex ELISA to an independent but clinically analogous cohort of males (n = 84, p= 0.002) and females (n = 95, p= 0.03).

Conclusions

The high Vitamin D status correlated with the serological upregulation of IGFBP-2 in males and IGFBP-3 in females with obesity and may constitute surrogate markers of risk reduction of cardiometabolic disease.

2044-4052
1-12
Al-Daghri, NM
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Manousopoulou, A
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Alokail, MS
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Yakout, S
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Alenad, A
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Garay-Baquero, DJ
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Fotopoulos, M
05714262-55c5-48a7-af57-397d5dd1cd6a
Teng, J
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Al-Attas, O
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Al-Saleh, Y
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Sabico, S
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Chrousos, GP
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Garbis, SD
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Al-Daghri, NM
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Manousopoulou, A
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Alokail, MS
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Yakout, S
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Alenad, A
9b01a9ff-c314-4948-a705-4eb25d43d410
Garay-Baquero, DJ
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Fotopoulos, M
05714262-55c5-48a7-af57-397d5dd1cd6a
Teng, J
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Al-Attas, O
4cb7dec9-090e-4718-9aab-36eae5c9514f
Al-Saleh, Y
b6a30c8e-441b-418f-9c77-cf61799fd2b1
Sabico, S
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Chrousos, GP
f44fc9ca-5043-4256-bf27-0af4620b8001
Garbis, SD
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Al-Daghri, NM, Manousopoulou, A, Alokail, MS, Yakout, S, Alenad, A, Garay-Baquero, DJ, Fotopoulos, M, Teng, J, Al-Attas, O, Al-Saleh, Y, Sabico, S, Chrousos, GP and Garbis, SD (2018) Sex-specific correlation of IGFBP-2 and IGFBP-3 with vitamin D status in adults with obesity: a cross-sectional serum proteomics study. Nutrition & Diabetes, 8, 1-12, [54]. (doi:10.1038/s41387-018-0063-8).

Record type: Article

Abstract

Objective

Subjects with low vitamin D levels are at risk of cardiometabolic disease. The aim of this study was to identify novel serological markers linking vitamin D status with cardiometabolic profile in non-diabetic adults with obesity.

Methods

For the discovery phase, we used quantitative serum proteomics in sex-matched, age-matched and BMI-matched subjects with obesity [BMI: 25–35 kg/m2] and low [25(OH)D < 50 nmol/L] vs. high vitamin D status [25(OH)D > 50 nmol/L] (n = 16). For the validation phase, we performed ELISA in a larger cohort with similar characteristics (n = 179).

Results

We identified 423 and 549 differentially expressed proteins in the high vs. low vitamin D groups of the male and female cohorts, respectively. The small molecule biochemistry protein networks and the glycolysis|gluconeogenesis pathway were significantly enriched in the DEPs of both sexes. As surrogate markers to these processes, the insulin-like growth factor binding protein -2  (IGFBP-2) was upregulated in males, whereas   IGFBP-3 was upregulated in females from the high Vitamin D status. This sex-specific trend  was confirmed using Luminex ELISA to an independent but clinically analogous cohort of males (n = 84, p= 0.002) and females (n = 95, p= 0.03).

Conclusions

The high Vitamin D status correlated with the serological upregulation of IGFBP-2 in males and IGFBP-3 in females with obesity and may constitute surrogate markers of risk reduction of cardiometabolic disease.

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Sex-specific correlation of IGFBP-2 and -3 with vitamin D status in adults with obesity A cross-sectional serum proteomics study - Accepted Manuscript
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Al-Daghri et al 2018 Nutrition Diabetes - Version of Record
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More information

Accepted/In Press date: 10 September 2018
e-pub ahead of print date: 4 October 2018
Published date: 4 October 2018

Identifiers

Local EPrints ID: 424117
URI: http://eprints.soton.ac.uk/id/eprint/424117
ISSN: 2044-4052
PURE UUID: 89625d8a-58a0-4495-8384-32b261fc5241
ORCID for DJ Garay-Baquero: ORCID iD orcid.org/0000-0002-9450-8504
ORCID for SD Garbis: ORCID iD orcid.org/0000-0002-1050-0805

Catalogue record

Date deposited: 04 Oct 2018 16:30
Last modified: 16 Jun 2021 01:43

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Contributors

Author: NM Al-Daghri
Author: A Manousopoulou
Author: MS Alokail
Author: S Yakout
Author: A Alenad
Author: DJ Garay-Baquero ORCID iD
Author: M Fotopoulos
Author: J Teng
Author: O Al-Attas
Author: Y Al-Saleh
Author: S Sabico
Author: GP Chrousos
Author: SD Garbis ORCID iD

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