PCR device with integrated thermal cycling and fluorescence detection elements

Bahrami, M., Melvin, Tracy, Wilkinson, James S. and Evans, A.G.R. (2005) PCR device with integrated thermal cycling and fluorescence detection elements. SPIE 2005 - Microtechnologies for the New Millennium, , Sevilla, Spain. 09 - 11 May 2005. (doi:10.1117/12.608646).

Record type: Conference or Workshop Item (Paper)

Abstract

In this paper, we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor, heaters, p-n diode and optical filter. The advantage of combining these in a single structure is that real time detection of DNA amplification will be possible using a small volume of the PCR solution. The photodiode is covered by a thin film optical filter in order to block out the light which is used to excite the fluorophore dyes. CdS is used for the first time for such a filter and the complete microfabrication process is described.

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