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Changes in miRNA gene expression during wound repair in differentiated normal human bronchial epithelium

Changes in miRNA gene expression during wound repair in differentiated normal human bronchial epithelium
Changes in miRNA gene expression during wound repair in differentiated normal human bronchial epithelium
Purpose. Airway epithelium acts as a protective barrier against the particles from the inhaled air. Damage to the epithelium may result in loss of the barrier function. Epithelial repair in response to injury requires complex mechanisms, such as microRNA, small noncoding molecules, to regulate the processes involved in wound repair. We aimed to establish if the microRNA gene expression profile is altered during the airway epithelial repair in differentiated cells. Methods. miRNA gene expression profile during the wound closure of differentiated normal human bronchial epithelium (NHBE) from one donor was analysed using quantitative real-time PCR. We have analysed the expression of 754 genes at five time points during a 48-hour period of epithelium repair using TaqMan Low Density Array. Results. We found out that 233 miRNA genes were expressed in normal human bronchial epithelium. Twenty miRNAs were differentially expressed during the wound repair process, but only one (miR-455-3p) showed significance after FDR adjustment (). Using STEM, we have identified two clusters of several miRNA genes with similar expression profile. Pathway enrichment analysis showed several significant signaling pathways altered during repair, mainly involved in cell cycle regulation, proliferation, migration, adhesion, and transcription regulation. Conclusions. miRNA expression profile is altered during airway epithelial repair of differentiated cells from one donor in response to mechanical injury in vitro, suggesting their potential role in wound repair.
2314-436X
1-8
Narożna, Beata
6b7fa4fb-58a3-4733-897e-be2ec6cc2c54
Langwiński, Wojciech
f4952744-0cc1-4451-bcc5-f2400e08fa61
Jackson, Claire
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Stachowiak, Zuzanna
586100df-5555-4de7-8b8a-c64bfd0ea9f1
Dmitrzak-węglarz, Monika
9eaea07a-4c26-42c3-9311-f5f975dd4f40
Szczepankiewicz, Aleksandra
54b47a55-9a85-44c8-b4b9-dea4a8d9fe73
Narożna, Beata
6b7fa4fb-58a3-4733-897e-be2ec6cc2c54
Langwiński, Wojciech
f4952744-0cc1-4451-bcc5-f2400e08fa61
Jackson, Claire
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Stachowiak, Zuzanna
586100df-5555-4de7-8b8a-c64bfd0ea9f1
Dmitrzak-węglarz, Monika
9eaea07a-4c26-42c3-9311-f5f975dd4f40
Szczepankiewicz, Aleksandra
54b47a55-9a85-44c8-b4b9-dea4a8d9fe73

Narożna, Beata, Langwiński, Wojciech, Jackson, Claire, Lackie, Peter M., Holloway, John W., Stachowiak, Zuzanna, Dmitrzak-węglarz, Monika and Szczepankiewicz, Aleksandra (2018) Changes in miRNA gene expression during wound repair in differentiated normal human bronchial epithelium. International Journal of Genomics, 2018, 1-8, [9093785]. (doi:10.1155/2018/9093785).

Record type: Article

Abstract

Purpose. Airway epithelium acts as a protective barrier against the particles from the inhaled air. Damage to the epithelium may result in loss of the barrier function. Epithelial repair in response to injury requires complex mechanisms, such as microRNA, small noncoding molecules, to regulate the processes involved in wound repair. We aimed to establish if the microRNA gene expression profile is altered during the airway epithelial repair in differentiated cells. Methods. miRNA gene expression profile during the wound closure of differentiated normal human bronchial epithelium (NHBE) from one donor was analysed using quantitative real-time PCR. We have analysed the expression of 754 genes at five time points during a 48-hour period of epithelium repair using TaqMan Low Density Array. Results. We found out that 233 miRNA genes were expressed in normal human bronchial epithelium. Twenty miRNAs were differentially expressed during the wound repair process, but only one (miR-455-3p) showed significance after FDR adjustment (). Using STEM, we have identified two clusters of several miRNA genes with similar expression profile. Pathway enrichment analysis showed several significant signaling pathways altered during repair, mainly involved in cell cycle regulation, proliferation, migration, adhesion, and transcription regulation. Conclusions. miRNA expression profile is altered during airway epithelial repair of differentiated cells from one donor in response to mechanical injury in vitro, suggesting their potential role in wound repair.

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9093785 - Version of Record
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More information

Accepted/In Press date: 8 August 2018
e-pub ahead of print date: 5 September 2018
Published date: 5 September 2018

Identifiers

Local EPrints ID: 424309
URI: http://eprints.soton.ac.uk/id/eprint/424309
ISSN: 2314-436X
PURE UUID: 1c48222c-25e3-48b6-8bce-488f80ada41e
ORCID for Peter M. Lackie: ORCID iD orcid.org/0000-0001-7138-3764
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 05 Oct 2018 11:36
Last modified: 22 Nov 2021 02:41

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Contributors

Author: Beata Narożna
Author: Wojciech Langwiński
Author: Claire Jackson
Author: Peter M. Lackie ORCID iD
Author: Zuzanna Stachowiak
Author: Monika Dmitrzak-węglarz
Author: Aleksandra Szczepankiewicz

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