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Biotechnological development of the marine microalgae Nannochloropsis gaditana

Biotechnological development of the marine microalgae Nannochloropsis gaditana
Biotechnological development of the marine microalgae Nannochloropsis gaditana
Marine microalgae are promising systems for biotechnology due to both their diversity and
ability to produce a vast array of metabolites, many of which are precursors in the production of
high-value products including fine chemicals and pharmaceuticals. Algae are also a potential
source of sustainable and carbon neutral biofuels although large-scale production systems are
currently in their infancy. To fully realize the potential of marine microalgae, non-model
biotechnologically relevant strains must be further improved using genetic engineering in order
to increase efficiency of production for commercial exploitation. However, the necessary
genetic tools for such approaches are in need of development on a species-to-species basis
before these needs can be met. This thesis describes the establishment and development of
principle molecular technologies in the oleaginous biofuel candidate alga Nannochloropsis
gaditana. An in situ fluorescent reporter system was established and used to characterize novel
genetic elements; including the main component of an inducible expression system capable of
precise temporal control of expression of transgenes through alteration of the growth media with
no negative effect on growth rates. Additionally, a promoter-trapping pipeline was designed for
the discovery of promoters that are highly expressed under specific environmental conditions.
Also in N. gaditana, high-throughput screening of mutant strains was used to isolate cell lines
with increased resistance to oxidative stress that exhibited improved growth in a variety of high
light treatments, as well as strains with increased lipid accumulation. These contributions to the
molecular toolkit for N. gaditana will further develop this phenotypically favorable strain
towards its amenability to highly sophisticated genetic engineering approaches. Several of the
technologies and strategies described may also have wider implications for improvement of
other species of marine microalgae for biotechnology.
University of Southampton
Jackson, Harry, Oliver
ea66eb4d-66c1-42aa-ace9-c624bc598263
Jackson, Harry, Oliver
ea66eb4d-66c1-42aa-ace9-c624bc598263
Bibby, Thomas
e04ea079-dd90-4ead-9840-00882de27ebd

Jackson, Harry, Oliver (2018) Biotechnological development of the marine microalgae Nannochloropsis gaditana. University of Southampton, Doctoral Thesis, 280pp.

Record type: Thesis (Doctoral)

Abstract

Marine microalgae are promising systems for biotechnology due to both their diversity and
ability to produce a vast array of metabolites, many of which are precursors in the production of
high-value products including fine chemicals and pharmaceuticals. Algae are also a potential
source of sustainable and carbon neutral biofuels although large-scale production systems are
currently in their infancy. To fully realize the potential of marine microalgae, non-model
biotechnologically relevant strains must be further improved using genetic engineering in order
to increase efficiency of production for commercial exploitation. However, the necessary
genetic tools for such approaches are in need of development on a species-to-species basis
before these needs can be met. This thesis describes the establishment and development of
principle molecular technologies in the oleaginous biofuel candidate alga Nannochloropsis
gaditana. An in situ fluorescent reporter system was established and used to characterize novel
genetic elements; including the main component of an inducible expression system capable of
precise temporal control of expression of transgenes through alteration of the growth media with
no negative effect on growth rates. Additionally, a promoter-trapping pipeline was designed for
the discovery of promoters that are highly expressed under specific environmental conditions.
Also in N. gaditana, high-throughput screening of mutant strains was used to isolate cell lines
with increased resistance to oxidative stress that exhibited improved growth in a variety of high
light treatments, as well as strains with increased lipid accumulation. These contributions to the
molecular toolkit for N. gaditana will further develop this phenotypically favorable strain
towards its amenability to highly sophisticated genetic engineering approaches. Several of the
technologies and strategies described may also have wider implications for improvement of
other species of marine microalgae for biotechnology.

Text
Jackson, Harry_PhD_Thesis_June_2018 - Author's Original
Available under License University of Southampton Thesis Licence.
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More information

Submitted date: 28 June 2018

Identifiers

Local EPrints ID: 424750
URI: http://eprints.soton.ac.uk/id/eprint/424750
PURE UUID: f3125341-98ce-452f-a7b9-e12645941fe5

Catalogue record

Date deposited: 05 Oct 2018 11:43
Last modified: 12 Dec 2021 08:53

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Contributors

Author: Harry, Oliver Jackson
Thesis advisor: Thomas Bibby

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