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Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms

Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms
Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms

This pilot study investigates a novel approach towards efficacy testing of antimicrobial cleaning agents; focusing primarily on hydrogen peroxide vapour (HPV). Contaminated surfaces are recognised modes of pathogen transmission within healthcare environments and increase the risk of pathogen acquisition in newly admitted patients. Studies have shown these pathogens can survive on surfaces for extended periods of time in spite of cleaning. This resilience is characteristic of biofilm formation and recent publications have identified their presence in hospitals. In this study, biofilm models comprised of multidrug-resistant organisms (MDROs) were generated using a drip flow reactor and exposed to HPV decontamination. The MDROs included Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Upon exposure, samples were periodically removed and enumerated to generate kill curves for each species. Consequently revealing any inherent resistances; such as catalase-producing organisms which expressed reduced susceptibility. Epifluorescence microscopy revealed an abundance of viable and non-viable microcolonies before and after decontamination, respectively. Greater than 6-Log10 reduction was achieved within a 100 minutes exposure time. This pilot study puts forward a potential methodology for testing antimicrobial agents against biofilms and supports the efficacy of HPV.

2045-2322
1-7
Watson, Fergus
c4c3927e-47bf-4448-a0c2-13a767e28ad8
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Chewins, John
07e26e61-1058-4872-8c54-500a0c4b3c26
Watson, Fergus
c4c3927e-47bf-4448-a0c2-13a767e28ad8
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, Sandra
86c1f41a-12b3-451c-9245-b1a21775e993
Chewins, John
07e26e61-1058-4872-8c54-500a0c4b3c26

Watson, Fergus, Keevil, Charles, Wilks, Sandra and Chewins, John (2018) Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms. Scientific Reports, 8 (1), 1-7, [12257]. (doi:10.1038/s41598-018-30706-0).

Record type: Article

Abstract

This pilot study investigates a novel approach towards efficacy testing of antimicrobial cleaning agents; focusing primarily on hydrogen peroxide vapour (HPV). Contaminated surfaces are recognised modes of pathogen transmission within healthcare environments and increase the risk of pathogen acquisition in newly admitted patients. Studies have shown these pathogens can survive on surfaces for extended periods of time in spite of cleaning. This resilience is characteristic of biofilm formation and recent publications have identified their presence in hospitals. In this study, biofilm models comprised of multidrug-resistant organisms (MDROs) were generated using a drip flow reactor and exposed to HPV decontamination. The MDROs included Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Upon exposure, samples were periodically removed and enumerated to generate kill curves for each species. Consequently revealing any inherent resistances; such as catalase-producing organisms which expressed reduced susceptibility. Epifluorescence microscopy revealed an abundance of viable and non-viable microcolonies before and after decontamination, respectively. Greater than 6-Log10 reduction was achieved within a 100 minutes exposure time. This pilot study puts forward a potential methodology for testing antimicrobial agents against biofilms and supports the efficacy of HPV.

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s41598-018-30706-0 - Version of Record
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More information

Accepted/In Press date: 30 July 2018
e-pub ahead of print date: 16 August 2018
Published date: 16 August 2018

Identifiers

Local EPrints ID: 424799
URI: http://eprints.soton.ac.uk/id/eprint/424799
ISSN: 2045-2322
PURE UUID: 3815de5f-d27a-4e52-adfd-aa0534e17246
ORCID for Charles Keevil: ORCID iD orcid.org/0000-0003-1917-7706
ORCID for Sandra Wilks: ORCID iD orcid.org/0000-0002-4134-9415

Catalogue record

Date deposited: 05 Oct 2018 11:46
Last modified: 18 Feb 2021 16:58

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