The choice of reference cell in the analysis of kinetic data using BIAcore
The choice of reference cell in the analysis of kinetic data using BIAcore
An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.
BIAcore, Kinetic constants, Reference cell, Surface plasmon resonance
70-80
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
15 June 1999
Ober, Raimund J.
31f4d47f-fb49-44f5-8ff6-87fc4aff3d36
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ober, Raimund J. and Ward, E. S.
(1999)
The choice of reference cell in the analysis of kinetic data using BIAcore.
Analytical Biochemistry, 271 (1), .
(doi:10.1006/abio.1999.4129).
Abstract
An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.
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Published date: 15 June 1999
Keywords:
BIAcore, Kinetic constants, Reference cell, Surface plasmon resonance
Identifiers
Local EPrints ID: 425019
URI: http://eprints.soton.ac.uk/id/eprint/425019
ISSN: 0003-2697
PURE UUID: a44d3db2-c4fd-4311-9b8c-fc27e9b3c275
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Date deposited: 09 Oct 2018 16:30
Last modified: 18 Mar 2024 03:48
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