The University of Southampton
University of Southampton Institutional Repository

A novel and efficient route for the isolation of antibodies that recognise T cell receptor Vαs

A novel and efficient route for the isolation of antibodies that recognise T cell receptor Vαs
A novel and efficient route for the isolation of antibodies that recognise T cell receptor Vαs

Studies of the T cell repertoire have been hindered by the lack of antibodies that recognise V region families, particularly for Vα regions. In this report, single chain Fv (scFv) fragments have been isolated that recognise both recombinant Vαs and native Vαs on the surface of T cells. Mice have been immunised with purified soluble T cell receptors (TCRs) and antibody heavy and light chain variable domain (VH and VL, respectively) genes isolated from splnocytes using the polymerase chain reaction (PCR). The VH and VL genes have been assembled as scFv gene libraries and a bacteriophage display system used to isolate scFvs that recognise a soluble Vα. Five scFvs have been purified and characterised in detail using enzyme-linked immunosorbent assays (ELISAs) and flow cytometry. Three of these five scFvs recognise native Vαs on the surface of T cell hybridomas. This method therefore offers a rapid route to the generation of scFvs that recognise native TCRs and can readily be extended to the production of anti-human TCR antibodies for use in therapy and diagnosis.

Antibodies, Phage display, Single chain Fv, T cell receptor
0161-5890
493-502
Popov, Sergei
15a37b99-253a-43a4-b825-19cd4e9b150e
Hubbard, J. Gregory
f9e5adb1-806e-49ea-aa3a-e292e4adeba9
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Popov, Sergei
15a37b99-253a-43a4-b825-19cd4e9b150e
Hubbard, J. Gregory
f9e5adb1-806e-49ea-aa3a-e292e4adeba9
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc

Popov, Sergei, Hubbard, J. Gregory and Ward, E. Sally (1996) A novel and efficient route for the isolation of antibodies that recognise T cell receptor Vαs. Molecular Immunology, 33 (6), 493-502. (doi:10.1016/0161-5890(96)00020-X).

Record type: Article

Abstract

Studies of the T cell repertoire have been hindered by the lack of antibodies that recognise V region families, particularly for Vα regions. In this report, single chain Fv (scFv) fragments have been isolated that recognise both recombinant Vαs and native Vαs on the surface of T cells. Mice have been immunised with purified soluble T cell receptors (TCRs) and antibody heavy and light chain variable domain (VH and VL, respectively) genes isolated from splnocytes using the polymerase chain reaction (PCR). The VH and VL genes have been assembled as scFv gene libraries and a bacteriophage display system used to isolate scFvs that recognise a soluble Vα. Five scFvs have been purified and characterised in detail using enzyme-linked immunosorbent assays (ELISAs) and flow cytometry. Three of these five scFvs recognise native Vαs on the surface of T cell hybridomas. This method therefore offers a rapid route to the generation of scFvs that recognise native TCRs and can readily be extended to the production of anti-human TCR antibodies for use in therapy and diagnosis.

Full text not available from this repository.

More information

Accepted/In Press date: 13 February 1996
Published date: April 1996
Keywords: Antibodies, Phage display, Single chain Fv, T cell receptor

Identifiers

Local EPrints ID: 425113
URI: https://eprints.soton.ac.uk/id/eprint/425113
ISSN: 0161-5890
PURE UUID: acb0fb9f-0a5f-442c-a27c-346d8977fd69
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 11 Oct 2018 16:30
Last modified: 22 May 2019 00:21

Export record

Altmetrics

Contributors

Author: Sergei Popov
Author: J. Gregory Hubbard
Author: E. Sally Ward ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×