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Functional expression of the MHC class I-related receptor, FcRn, in endothelial cells of mice

Functional expression of the MHC class I-related receptor, FcRn, in endothelial cells of mice
Functional expression of the MHC class I-related receptor, FcRn, in endothelial cells of mice

Our recent data indicate that the MHC class I-related receptor, FcRn, plays a role in regulating serum IgG levels, in addition to its known role in transferring IgG from mother to young. In the current study, the distribution of FcRn in adult mice has been investigated using several approaches. First, tissue distribution of anti-FcRn F(ab')2, murine IgG1 and recombinant, IgG1-derived Fc-hinge fragments has been analyzed, and these FcRn binding proteins localize predominantly in skin and muscle with lesser amounts in liver and adipose tissue. Second, histochemical analyses of muscle and liver with anti-FcRn F(ab')2 indicate that FcRn is expressed in the endothelium of small arterioles and capillaries, but not in larger vessels such as the central vein and portal vasculature. Third, immunoprecipitation and immunofluorescence studies of cultured murine endothelial cells show that functional FcRn is expressed in these cells, and is located within vesicular structures in the cytosol and not on the membrane. Taken together the data demonstrate that FcRn is expressed in functionally active form in endothelial cells, indicating that these cells are a possible site at which serum IgG homeostasis is maintained.

Endothelial cell, FcRn, IgG
0953-8178
1289-1298
Borvak, Jozef
0756de83-6795-49ab-9df5-07e3dc3ae05e
Richardson, James
397c7313-6a03-4cb7-84df-4fb4314b579d
Medesan, Cornel
0de1d9e4-c8d9-4896-bcca-63f8b24e8f2c
Antohe, Felicia
7723c6d9-3905-4016-a6ff-b18ac5d575f9
Radu, Caius
7b0cdea8-4ad8-4c89-89f7-eb6083504c1c
Simionescu, Maya
dc8c225c-558d-49c7-b3cf-d1c6befc79fb
Ghetie, Victor
b7b50946-2bd9-4896-b841-6bbfba8237c2
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Borvak, Jozef
0756de83-6795-49ab-9df5-07e3dc3ae05e
Richardson, James
397c7313-6a03-4cb7-84df-4fb4314b579d
Medesan, Cornel
0de1d9e4-c8d9-4896-bcca-63f8b24e8f2c
Antohe, Felicia
7723c6d9-3905-4016-a6ff-b18ac5d575f9
Radu, Caius
7b0cdea8-4ad8-4c89-89f7-eb6083504c1c
Simionescu, Maya
dc8c225c-558d-49c7-b3cf-d1c6befc79fb
Ghetie, Victor
b7b50946-2bd9-4896-b841-6bbfba8237c2
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc

Borvak, Jozef, Richardson, James, Medesan, Cornel, Antohe, Felicia, Radu, Caius, Simionescu, Maya, Ghetie, Victor and Ward, E. Sally (1998) Functional expression of the MHC class I-related receptor, FcRn, in endothelial cells of mice. International Immunology, 10 (9), 1289-1298. (doi:10.1093/intimm/10.9.1289).

Record type: Article

Abstract

Our recent data indicate that the MHC class I-related receptor, FcRn, plays a role in regulating serum IgG levels, in addition to its known role in transferring IgG from mother to young. In the current study, the distribution of FcRn in adult mice has been investigated using several approaches. First, tissue distribution of anti-FcRn F(ab')2, murine IgG1 and recombinant, IgG1-derived Fc-hinge fragments has been analyzed, and these FcRn binding proteins localize predominantly in skin and muscle with lesser amounts in liver and adipose tissue. Second, histochemical analyses of muscle and liver with anti-FcRn F(ab')2 indicate that FcRn is expressed in the endothelium of small arterioles and capillaries, but not in larger vessels such as the central vein and portal vasculature. Third, immunoprecipitation and immunofluorescence studies of cultured murine endothelial cells show that functional FcRn is expressed in these cells, and is located within vesicular structures in the cytosol and not on the membrane. Taken together the data demonstrate that FcRn is expressed in functionally active form in endothelial cells, indicating that these cells are a possible site at which serum IgG homeostasis is maintained.

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More information

Published date: September 1998
Keywords: Endothelial cell, FcRn, IgG

Identifiers

Local EPrints ID: 425124
URI: http://eprints.soton.ac.uk/id/eprint/425124
ISSN: 0953-8178
PURE UUID: 32b2ca9e-23a0-4d0c-9908-9008150bc6c6
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 11 Oct 2018 16:30
Last modified: 15 Jun 2022 01:52

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Contributors

Author: Jozef Borvak
Author: James Richardson
Author: Cornel Medesan
Author: Felicia Antohe
Author: Caius Radu
Author: Maya Simionescu
Author: Victor Ghetie
Author: E. Sally Ward ORCID iD

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