Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1
Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1
The interaction of mouse IgG1 or IgG1-derived Fc fragment with recombinant, insect cell expressed mouse FcRn has been analyzed using sedimentation equilibrium. This results in a model for the interaction in which the two binding sites for FcRn on Fc or IgG1 have significantly different affinities with macroscopic binding constants of <130 nM and 6 μM. This data indicates the formation of an asymmetric FcRn:Fc (or IgG1):FcRn complex which is consistent with earlier suggestions that for this form of recombinant FcRn, binding to IgG1 or Fc does not result in a symmetric 2:1 complex in which both binding sites are equivalent. (C) 2000 Elsevier Science Ltd.
Murine IgG1, Neonatal Fc receptor, Sedimentation equilibrium
1117-1125
Schuck, Peter
d54e80e0-8644-409a-baf9-893eda952b5d
Radu, Caius G.
7b0cdea8-4ad8-4c89-89f7-eb6083504c1c
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
October 1999
Schuck, Peter
d54e80e0-8644-409a-baf9-893eda952b5d
Radu, Caius G.
7b0cdea8-4ad8-4c89-89f7-eb6083504c1c
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Schuck, Peter, Radu, Caius G. and Ward, E. Sally
(1999)
Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1.
Molecular Immunology, 36 (15-16), .
(doi:10.1016/S0161-5890(99)00093-0).
Abstract
The interaction of mouse IgG1 or IgG1-derived Fc fragment with recombinant, insect cell expressed mouse FcRn has been analyzed using sedimentation equilibrium. This results in a model for the interaction in which the two binding sites for FcRn on Fc or IgG1 have significantly different affinities with macroscopic binding constants of <130 nM and 6 μM. This data indicates the formation of an asymmetric FcRn:Fc (or IgG1):FcRn complex which is consistent with earlier suggestions that for this form of recombinant FcRn, binding to IgG1 or Fc does not result in a symmetric 2:1 complex in which both binding sites are equivalent. (C) 2000 Elsevier Science Ltd.
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Accepted/In Press date: 8 June 1999
Published date: October 1999
Keywords:
Murine IgG1, Neonatal Fc receptor, Sedimentation equilibrium
Identifiers
Local EPrints ID: 425129
URI: http://eprints.soton.ac.uk/id/eprint/425129
ISSN: 0161-5890
PURE UUID: 4c742a67-df28-4258-8e4f-eaf23211e228
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Date deposited: 11 Oct 2018 16:30
Last modified: 18 Mar 2024 03:48
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Author:
Peter Schuck
Author:
Caius G. Radu
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