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Anti-Vβ8 antibodies induce and maintain staphylococcal enterotoxin B-triggered Vβ8+ T cell anergy

Anti-Vβ8 antibodies induce and maintain staphylococcal enterotoxin B-triggered Vβ8+ T cell anergy
Anti-Vβ8 antibodies induce and maintain staphylococcal enterotoxin B-triggered Vβ8+ T cell anergy

The mechanism involved in the maintenance of staphylococcal enterotoxin B (SEB)-induced T cell anergy is poorly understood. We demonstrated earlier that B cells play an important role in the maintenance of SEB-induced T cell anergy in vivo and in vitro. Here, we demonstrate that B cells are not essential in SEB-induced T cell activation, but are important for the maintenance of T cell memory phenotype and anergy in vivo. Studying the activated B cell repertoire, we observe that SEB treatment increases serum anti-Vβ8 antibody titer as detected by enzyme-linked immunosorbent assay using soluble Vβ8 chains as antigens, and by staining of a Vβ8-expressing thymoma. These antibodies disappear gradually after immunization with SEB, whereas the capacity of the T cells to respond to SEB in vitro is restored. Anti-Vβ8 monoclonal antibody treatment causes Vβ8+ T cell unresponsiveness to SEB in vitro (anergy), without affecting CD4Vβ8+ T cell frequency. Together, these results suggest a new mechanism to explain the maintenance of SEB-induced T cell anergy, which is depen dent on B cells and on anti-Vβ8 antibody that specifically interacts with Vβ8+ T cells.

Anergy, Antibody, Staphylococcal enterotoxin B, T cell receptor
0014-2980
437-445
Aroeira, Luiz Stark
df486208-4854-4758-a91a-b31f58b3bf1c
Mouton, Concepción G.
cacdd5b8-f0b1-41cb-874f-824e38db91ba
Toran, José L.
cf2cc459-ef20-4701-9bd5-193047fd251f
Ward, Elizabeth Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Martínez-A, Carlos
f978d2c8-30a4-4698-9eb2-3e8c491bed68
Aroeira, Luiz Stark
df486208-4854-4758-a91a-b31f58b3bf1c
Mouton, Concepción G.
cacdd5b8-f0b1-41cb-874f-824e38db91ba
Toran, José L.
cf2cc459-ef20-4701-9bd5-193047fd251f
Ward, Elizabeth Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Martínez-A, Carlos
f978d2c8-30a4-4698-9eb2-3e8c491bed68

Aroeira, Luiz Stark, Mouton, Concepción G., Toran, José L., Ward, Elizabeth Sally and Martínez-A, Carlos (1999) Anti-Vβ8 antibodies induce and maintain staphylococcal enterotoxin B-triggered Vβ8+ T cell anergy. European Journal of Immunology, 29 (2), 437-445. (doi:10.1002/(SICI)1521-4141(199902)29:02<437::AID-IMMU437>3.0.CO;2-A).

Record type: Article

Abstract

The mechanism involved in the maintenance of staphylococcal enterotoxin B (SEB)-induced T cell anergy is poorly understood. We demonstrated earlier that B cells play an important role in the maintenance of SEB-induced T cell anergy in vivo and in vitro. Here, we demonstrate that B cells are not essential in SEB-induced T cell activation, but are important for the maintenance of T cell memory phenotype and anergy in vivo. Studying the activated B cell repertoire, we observe that SEB treatment increases serum anti-Vβ8 antibody titer as detected by enzyme-linked immunosorbent assay using soluble Vβ8 chains as antigens, and by staining of a Vβ8-expressing thymoma. These antibodies disappear gradually after immunization with SEB, whereas the capacity of the T cells to respond to SEB in vitro is restored. Anti-Vβ8 monoclonal antibody treatment causes Vβ8+ T cell unresponsiveness to SEB in vitro (anergy), without affecting CD4Vβ8+ T cell frequency. Together, these results suggest a new mechanism to explain the maintenance of SEB-induced T cell anergy, which is depen dent on B cells and on anti-Vβ8 antibody that specifically interacts with Vβ8+ T cells.

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More information

Accepted/In Press date: 30 October 1998
e-pub ahead of print date: 5 February 1999
Published date: February 1999
Keywords: Anergy, Antibody, Staphylococcal enterotoxin B, T cell receptor

Identifiers

Local EPrints ID: 425131
URI: http://eprints.soton.ac.uk/id/eprint/425131
ISSN: 0014-2980
PURE UUID: bc75f849-19cf-440c-bb35-e04fe429724d
ORCID for Elizabeth Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 11 Oct 2018 16:30
Last modified: 07 Oct 2020 02:22

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Contributors

Author: Luiz Stark Aroeira
Author: Concepción G. Mouton
Author: José L. Toran
Author: Carlos Martínez-A

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