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Crystal structure of a T cell receptor Vα11 (AV11S5) domain: New canonical forms for the first and second complementarity determining regions

Crystal structure of a T cell receptor Vα11 (AV11S5) domain: New canonical forms for the first and second complementarity determining regions
Crystal structure of a T cell receptor Vα11 (AV11S5) domain: New canonical forms for the first and second complementarity determining regions

We describe the X-ray crystallographic structure of a murine T cell receptor (TCR) Vα domain ("Vα85.33"; AV11S5-AJ17) to 1.85 Å resolution. The Vα85.33 domain is derived from a TCR that recognizes a type II collagen peptide associated with the murine major histocompatibility complex (MHC) class II molecule, I-Aq. Vα85.33 packs as a Vα-Vα homodimer with a highly symmetric monomer-monomer interface. The first and second complementarity determining regions (CDR1 and CDR2) of this Vα are shorter than the CDRs corresponding to the majority of other Vα gene families, and three-dimensional structures of CDRs of these lengths have not been described previously. The CDR1 and CDR2 therefore represent new canonical forms that could serve as templates for AV11 family members. CDR3 of the Vα85.33 domain is highly flexible and this is consistent with plasticity of this region of the TCR. The fourth hypervariable loop (HV4α) of AV11 and AV10 family members is one residue longer than that of other HV4α regions and shows a high degree of flexibility. The increase in length results in a distinct disposition of the conserved residue Lys68, which has been shown in other studies to play a role in antigen recognition. The X-ray structure of Vα85.33 extends the database of canonical forms for CDR1 and CDR2, and has implications for antigen recognition by TCRs that contain related Vα domains.

Antigen recognition, Canonical form, Complementarity determining region, T cell receptor Vα domain, X-ray crystallography
0022-2836
689-698
Machius, Mischa
87c52254-f088-4e7d-87b9-9ccbfddebd7d
Cianga, Petru
0c3783a1-22f2-40b9-80a6-9a580fef2477
Deisenhofer, Johann
73661465-56e7-4200-9260-5f782047e122
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Machius, Mischa
87c52254-f088-4e7d-87b9-9ccbfddebd7d
Cianga, Petru
0c3783a1-22f2-40b9-80a6-9a580fef2477
Deisenhofer, Johann
73661465-56e7-4200-9260-5f782047e122
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc

Machius, Mischa, Cianga, Petru, Deisenhofer, Johann and Ward, E. Sally (2001) Crystal structure of a T cell receptor Vα11 (AV11S5) domain: New canonical forms for the first and second complementarity determining regions. Journal of Molecular Biology, 310 (4), 689-698. (doi:10.1006/jmbi.2001.4794).

Record type: Article

Abstract

We describe the X-ray crystallographic structure of a murine T cell receptor (TCR) Vα domain ("Vα85.33"; AV11S5-AJ17) to 1.85 Å resolution. The Vα85.33 domain is derived from a TCR that recognizes a type II collagen peptide associated with the murine major histocompatibility complex (MHC) class II molecule, I-Aq. Vα85.33 packs as a Vα-Vα homodimer with a highly symmetric monomer-monomer interface. The first and second complementarity determining regions (CDR1 and CDR2) of this Vα are shorter than the CDRs corresponding to the majority of other Vα gene families, and three-dimensional structures of CDRs of these lengths have not been described previously. The CDR1 and CDR2 therefore represent new canonical forms that could serve as templates for AV11 family members. CDR3 of the Vα85.33 domain is highly flexible and this is consistent with plasticity of this region of the TCR. The fourth hypervariable loop (HV4α) of AV11 and AV10 family members is one residue longer than that of other HV4α regions and shows a high degree of flexibility. The increase in length results in a distinct disposition of the conserved residue Lys68, which has been shown in other studies to play a role in antigen recognition. The X-ray structure of Vα85.33 extends the database of canonical forms for CDR1 and CDR2, and has implications for antigen recognition by TCRs that contain related Vα domains.

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More information

Accepted/In Press date: 22 May 2001
Published date: 20 July 2001
Keywords: Antigen recognition, Canonical form, Complementarity determining region, T cell receptor Vα domain, X-ray crystallography

Identifiers

Local EPrints ID: 425134
URI: http://eprints.soton.ac.uk/id/eprint/425134
ISSN: 0022-2836
PURE UUID: 4f0b88a0-cd51-4085-bdeb-201942af7bff
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 11 Oct 2018 16:30
Last modified: 07 Oct 2020 02:22

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Contributors

Author: Mischa Machius
Author: Petru Cianga
Author: Johann Deisenhofer
Author: E. Sally Ward ORCID iD

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