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Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: Functional expression of FcRn in the mammalian lung

Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: Functional expression of FcRn in the mammalian lung
Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: Functional expression of FcRn in the mammalian lung

Mucosal secretions of the human gastrointestinal, respiratory, and genital tracts contain the immunoglobulins (Ig)G and secretory IgA (sIgA) that function together in host defense. Exactly how IgG crosses epithelial barriers to function in mucosal immunity remains unknown. Here, we test the idea that the MHC class I-related Fc-receptor, FcRn, transports IgG across the mucosal surface of the human and mouse lung from lumen to serosa. We find that bronchial epithelial cells of the human, nonhuman primate, and mouse, express FcRn in adult-life, and demonstrate FcRn-dependent absorption of a bioactive Fc-fusion protein across the respiratory epithelium of the mouse in vivo. Thus, IgG, like dimeric IgA, can cross epithelial barriers by receptor-mediated transcytosis in adult animals. These data show that mucosal surfaces that express FcRn reabsorb IgG and explain a mechanism by which IgG may act in immune surveillance to retrieve lumenal antigens for processing in the lamina propria or systemically.

Epithelial cells, FcRn, IgG, Lung, Transcytosis
0022-1007
303-310
Spiekermann, Gerburg M.
1efbb8db-71e6-4ed3-b2f6-5e0d8edcd6aa
Finn, Patricia W.
e13be10f-a570-4f2b-abfe-1ba2258c314b
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Dumont, Jennifer
e52ced2c-ff5a-4c81-bc93-0286040313e3
Dickinson, Bonny L.
ddc8bb22-71d6-49db-a8c1-7b2d92055e02
Blumberg, Richard S.
3fe5de1c-ff8a-41bb-9009-f27a37bd1bc5
Lencer, Wayne I.
6191ed36-e2a0-4d5d-a6fd-a972877cff5f
Spiekermann, Gerburg M.
1efbb8db-71e6-4ed3-b2f6-5e0d8edcd6aa
Finn, Patricia W.
e13be10f-a570-4f2b-abfe-1ba2258c314b
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Dumont, Jennifer
e52ced2c-ff5a-4c81-bc93-0286040313e3
Dickinson, Bonny L.
ddc8bb22-71d6-49db-a8c1-7b2d92055e02
Blumberg, Richard S.
3fe5de1c-ff8a-41bb-9009-f27a37bd1bc5
Lencer, Wayne I.
6191ed36-e2a0-4d5d-a6fd-a972877cff5f

Spiekermann, Gerburg M., Finn, Patricia W., Ward, E. Sally, Dumont, Jennifer, Dickinson, Bonny L., Blumberg, Richard S. and Lencer, Wayne I. (2002) Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: Functional expression of FcRn in the mammalian lung. Journal of Experimental Medicine, 196 (3), 303-310. (doi:10.1084/jem.20020400).

Record type: Article

Abstract

Mucosal secretions of the human gastrointestinal, respiratory, and genital tracts contain the immunoglobulins (Ig)G and secretory IgA (sIgA) that function together in host defense. Exactly how IgG crosses epithelial barriers to function in mucosal immunity remains unknown. Here, we test the idea that the MHC class I-related Fc-receptor, FcRn, transports IgG across the mucosal surface of the human and mouse lung from lumen to serosa. We find that bronchial epithelial cells of the human, nonhuman primate, and mouse, express FcRn in adult-life, and demonstrate FcRn-dependent absorption of a bioactive Fc-fusion protein across the respiratory epithelium of the mouse in vivo. Thus, IgG, like dimeric IgA, can cross epithelial barriers by receptor-mediated transcytosis in adult animals. These data show that mucosal surfaces that express FcRn reabsorb IgG and explain a mechanism by which IgG may act in immune surveillance to retrieve lumenal antigens for processing in the lamina propria or systemically.

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More information

Accepted/In Press date: 13 June 2002
e-pub ahead of print date: 29 July 2002
Published date: 5 August 2002
Keywords: Epithelial cells, FcRn, IgG, Lung, Transcytosis

Identifiers

Local EPrints ID: 425143
URI: http://eprints.soton.ac.uk/id/eprint/425143
ISSN: 0022-1007
PURE UUID: a3f34188-d854-4215-abe5-322da56de4f8
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 11 Oct 2018 16:30
Last modified: 16 Mar 2024 04:37

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Contributors

Author: Gerburg M. Spiekermann
Author: Patricia W. Finn
Author: E. Sally Ward ORCID iD
Author: Jennifer Dumont
Author: Bonny L. Dickinson
Author: Richard S. Blumberg
Author: Wayne I. Lencer

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