Antigen specificity of clonally expanded and receptor edited cerebrospinal fluid B cells from patients with relapsing remitting MS
Antigen specificity of clonally expanded and receptor edited cerebrospinal fluid B cells from patients with relapsing remitting MS
We re-engineered the immunoglobulin rearrangements from clonally expanded CSF B cells of three Multiple Sclerosis patients as Fab fragments, and used three methods to test for their antigen (Ag) specificity. Nine out of ten Fab fragments were reactive to Myelin Basic Protein (MBP). The one Fab that did not react to MBP was a product of receptor editing. Two of the nine MBP reactive Fabs were also reactive to GFAP and CNPase, indicating that these clones were polyreactive. Targeting the mechanisms that allow these self-reactive B cells to reside in the CSF of MS patients may prove to be a potent immunotherapeutic strategy.
Antibodies, Antigen specificity, B lymphocytes, Gene rearrangement, Multiple sclerosis
164-176
Lambracht-Washington, Doris
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O'Connor, Kevin C.
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Cameron, Elizabeth M.
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Jowdry, Andrea
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Ward, E. Sally
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Frohman, Elliot
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Racke, Michael K.
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Monson, Nancy L.
2fe3f326-3515-4c3f-bbce-f6f043bb5f61
May 2007
Lambracht-Washington, Doris
e8c92c2c-d936-45d4-a7bd-fa648de06e81
O'Connor, Kevin C.
a549dc1e-c261-4f8c-b3f5-9ad41ef773c7
Cameron, Elizabeth M.
117633c8-2dc1-4e3a-ab1a-2b45c17789c3
Jowdry, Andrea
d75fe4b1-2275-4396-9d21-13c50ab2a373
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Frohman, Elliot
9f797b36-3012-46d0-b48f-0bbdd9c30f56
Racke, Michael K.
fd372156-c5df-402d-82de-8ecc3a4bce3f
Monson, Nancy L.
2fe3f326-3515-4c3f-bbce-f6f043bb5f61
Lambracht-Washington, Doris, O'Connor, Kevin C., Cameron, Elizabeth M., Jowdry, Andrea, Ward, E. Sally, Frohman, Elliot, Racke, Michael K. and Monson, Nancy L.
(2007)
Antigen specificity of clonally expanded and receptor edited cerebrospinal fluid B cells from patients with relapsing remitting MS.
Journal of Neuroimmunology, 186 (1-2), .
(doi:10.1016/j.jneuroim.2007.03.002).
Abstract
We re-engineered the immunoglobulin rearrangements from clonally expanded CSF B cells of three Multiple Sclerosis patients as Fab fragments, and used three methods to test for their antigen (Ag) specificity. Nine out of ten Fab fragments were reactive to Myelin Basic Protein (MBP). The one Fab that did not react to MBP was a product of receptor editing. Two of the nine MBP reactive Fabs were also reactive to GFAP and CNPase, indicating that these clones were polyreactive. Targeting the mechanisms that allow these self-reactive B cells to reside in the CSF of MS patients may prove to be a potent immunotherapeutic strategy.
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Accepted/In Press date: 2 March 2007
e-pub ahead of print date: 23 April 2007
Published date: May 2007
Keywords:
Antibodies, Antigen specificity, B lymphocytes, Gene rearrangement, Multiple sclerosis
Identifiers
Local EPrints ID: 425156
URI: http://eprints.soton.ac.uk/id/eprint/425156
ISSN: 0165-5728
PURE UUID: d42685e3-c2e1-425d-b7bc-e5a46707ff2e
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Date deposited: 11 Oct 2018 16:30
Last modified: 16 Mar 2024 04:37
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Contributors
Author:
Doris Lambracht-Washington
Author:
Kevin C. O'Connor
Author:
Elizabeth M. Cameron
Author:
Andrea Jowdry
Author:
Elliot Frohman
Author:
Michael K. Racke
Author:
Nancy L. Monson
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