Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis
Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis
Plasmid analysis of Bacillus thuringiensis var. israelensis revealed the presence of at least 9 plasmids. A high frequency of plasmid loss occurred when this organism was grown at elevated temperature (42°C). Analysis of over 100 isolates cured of one or more plasmids by this method revealed that loss of a 72 MDa plasmid was invariably accompanied by loss of the ability to synthesize the insecticidal δ-endotoxin protein. Deletion of any of the other plasmids had no effect on δ-endotoxin production. These results indicate the presence of a plasmid-coded copy of the structural gene for the insecticidal δ-endotoxin in B. thuringiensis var. israelensis.
Bacillus thuringiensis var. israelensis, Gene, Insecticide, Plasmid, Plasmid curing, δ-Endotoxin
45-49
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
11 July 1983
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
Ward, E. S. and Ellar, D. J.
(1983)
Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis.
FEBS Letters, 158 (1), .
(doi:10.1016/0014-5793(83)80673-5).
Abstract
Plasmid analysis of Bacillus thuringiensis var. israelensis revealed the presence of at least 9 plasmids. A high frequency of plasmid loss occurred when this organism was grown at elevated temperature (42°C). Analysis of over 100 isolates cured of one or more plasmids by this method revealed that loss of a 72 MDa plasmid was invariably accompanied by loss of the ability to synthesize the insecticidal δ-endotoxin protein. Deletion of any of the other plasmids had no effect on δ-endotoxin production. These results indicate the presence of a plasmid-coded copy of the structural gene for the insecticidal δ-endotoxin in B. thuringiensis var. israelensis.
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Published date: 11 July 1983
Keywords:
Bacillus thuringiensis var. israelensis, Gene, Insecticide, Plasmid, Plasmid curing, δ-Endotoxin
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Local EPrints ID: 425243
URI: http://eprints.soton.ac.uk/id/eprint/425243
ISSN: 0014-5793
PURE UUID: 74e78dee-4c27-4e2a-a788-2085c206b52a
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Date deposited: 12 Oct 2018 16:30
Last modified: 16 Mar 2024 04:37
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Author:
D. J. Ellar
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