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Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis

Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis
Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis

Plasmid analysis of Bacillus thuringiensis var. israelensis revealed the presence of at least 9 plasmids. A high frequency of plasmid loss occurred when this organism was grown at elevated temperature (42°C). Analysis of over 100 isolates cured of one or more plasmids by this method revealed that loss of a 72 MDa plasmid was invariably accompanied by loss of the ability to synthesize the insecticidal δ-endotoxin protein. Deletion of any of the other plasmids had no effect on δ-endotoxin production. These results indicate the presence of a plasmid-coded copy of the structural gene for the insecticidal δ-endotoxin in B. thuringiensis var. israelensis.

Bacillus thuringiensis var. israelensis, Gene, Insecticide, Plasmid, Plasmid curing, δ-Endotoxin
0014-5793
45-49
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199

Ward, E. S. and Ellar, D. J. (1983) Assignment of the δ-endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis. FEBS Letters, 158 (1), 45-49. (doi:10.1016/0014-5793(83)80673-5).

Record type: Article

Abstract

Plasmid analysis of Bacillus thuringiensis var. israelensis revealed the presence of at least 9 plasmids. A high frequency of plasmid loss occurred when this organism was grown at elevated temperature (42°C). Analysis of over 100 isolates cured of one or more plasmids by this method revealed that loss of a 72 MDa plasmid was invariably accompanied by loss of the ability to synthesize the insecticidal δ-endotoxin protein. Deletion of any of the other plasmids had no effect on δ-endotoxin production. These results indicate the presence of a plasmid-coded copy of the structural gene for the insecticidal δ-endotoxin in B. thuringiensis var. israelensis.

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More information

Published date: 11 July 1983
Keywords: Bacillus thuringiensis var. israelensis, Gene, Insecticide, Plasmid, Plasmid curing, δ-Endotoxin

Identifiers

Local EPrints ID: 425243
URI: https://eprints.soton.ac.uk/id/eprint/425243
ISSN: 0014-5793
PURE UUID: 74e78dee-4c27-4e2a-a788-2085c206b52a
ORCID for E. S. Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 12 Oct 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: E. S. Ward ORCID iD
Author: D. J. Ellar

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