Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis
Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis
The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensis δ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72-74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.
(DNA homology, Bacillus thuringiensis, Plasmid), δ-Endotoxin gene
195-199
Earp, David J.
ffaca7c8-e214-4e28-9550-5f1d1b343cb1
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, David J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
1 July 1987
Earp, David J.
ffaca7c8-e214-4e28-9550-5f1d1b343cb1
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, David J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
Earp, David J., Ward, E. Sally and Ellar, David J.
(1987)
Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis.
FEMS Microbiology Letters, 42 (2-3), .
(doi:10.1111/j.1574-6968.1987.tb02071.x).
Abstract
The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensis δ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72-74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.
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Published date: 1 July 1987
Keywords:
(DNA homology, Bacillus thuringiensis, Plasmid), δ-Endotoxin gene
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Local EPrints ID: 425245
URI: http://eprints.soton.ac.uk/id/eprint/425245
ISSN: 0378-1097
PURE UUID: d3d07546-5756-4d1d-80a7-766f0bce3fb1
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Date deposited: 12 Oct 2018 16:30
Last modified: 18 Mar 2024 03:48
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Author:
David J. Earp
Author:
David J. Ellar
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