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Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis

Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis
Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis

The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensis δ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72-74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.

(DNA homology, Bacillus thuringiensis, Plasmid), δ-Endotoxin gene
0378-1097
195-199
Earp, David J.
ffaca7c8-e214-4e28-9550-5f1d1b343cb1
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, David J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
Earp, David J.
ffaca7c8-e214-4e28-9550-5f1d1b343cb1
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, David J.
afb465a4-5ccc-4ac8-8dc1-38789156951d

Earp, David J., Ward, E. Sally and Ellar, David J. (1987) Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis. FEMS Microbiology Letters, 42 (2-3), 195-199. (doi:10.1111/j.1574-6968.1987.tb02071.x).

Record type: Article

Abstract

The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensis δ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72-74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.

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More information

Published date: 1 July 1987
Keywords: (DNA homology, Bacillus thuringiensis, Plasmid), δ-Endotoxin gene

Identifiers

Local EPrints ID: 425245
URI: https://eprints.soton.ac.uk/id/eprint/425245
ISSN: 0378-1097
PURE UUID: d3d07546-5756-4d1d-80a7-766f0bce3fb1
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 12 Oct 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: David J. Earp
Author: E. Sally Ward ORCID iD
Author: David J. Ellar

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