Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli
Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli
The nucleotide sequence of a 1408 base-pair DNA fragment encoding the insecticidal 27,340 Mr δ-endotoxin of Bacillus thuringiensis var. israelensis has been determined by analysis of a recombinant plasmid from Escherichia coli. The hydropathy plot of the protein shows it to be highly hydrophobic, consistent with a postulated cytolytic mechanism of action for the toxin. In addition, the δ-endotoxin transcriptional start points that are used in B. thuringiensis and an E. coli recombinant have been determined. In B. thuringiensis var. israelensis, transcription initiates from a single start point, and gene-specific transcripts are not observed before stage II of sporulation. This is the stage at which δ-endotoxin antigen is first detected, indicating that control of expression is primarily at the transcriptional level for this protein. Analysis of gene-specific transcription in E. coli indicates that at least three start points are utilized in this organism. Interestingly, the highest level of δ-endotoxin mRNA is seen during mid-exponential growth of E. coli and the level appears to decrease as the cells enter the stationary phase of growth.
1-11
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
5 September 1986
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
afb465a4-5ccc-4ac8-8dc1-38789156951d
Ward, E. S. and Ellar, D. J.
(1986)
Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli.
Journal of Molecular Biology, 191 (1), .
(doi:10.1016/0022-2836(86)90417-1).
Abstract
The nucleotide sequence of a 1408 base-pair DNA fragment encoding the insecticidal 27,340 Mr δ-endotoxin of Bacillus thuringiensis var. israelensis has been determined by analysis of a recombinant plasmid from Escherichia coli. The hydropathy plot of the protein shows it to be highly hydrophobic, consistent with a postulated cytolytic mechanism of action for the toxin. In addition, the δ-endotoxin transcriptional start points that are used in B. thuringiensis and an E. coli recombinant have been determined. In B. thuringiensis var. israelensis, transcription initiates from a single start point, and gene-specific transcripts are not observed before stage II of sporulation. This is the stage at which δ-endotoxin antigen is first detected, indicating that control of expression is primarily at the transcriptional level for this protein. Analysis of gene-specific transcription in E. coli indicates that at least three start points are utilized in this organism. Interestingly, the highest level of δ-endotoxin mRNA is seen during mid-exponential growth of E. coli and the level appears to decrease as the cells enter the stationary phase of growth.
This record has no associated files available for download.
More information
Published date: 5 September 1986
Identifiers
Local EPrints ID: 425247
URI: http://eprints.soton.ac.uk/id/eprint/425247
ISSN: 0022-2836
PURE UUID: 111c6b0b-0848-47f7-9315-6cc62d7067eb
Catalogue record
Date deposited: 12 Oct 2018 16:30
Last modified: 18 Mar 2024 03:48
Export record
Altmetrics
Contributors
Author:
D. J. Ellar
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
