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Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli

Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli
Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli

The nucleotide sequence of a 1408 base-pair DNA fragment encoding the insecticidal 27,340 Mr δ-endotoxin of Bacillus thuringiensis var. israelensis has been determined by analysis of a recombinant plasmid from Escherichia coli. The hydropathy plot of the protein shows it to be highly hydrophobic, consistent with a postulated cytolytic mechanism of action for the toxin. In addition, the δ-endotoxin transcriptional start points that are used in B. thuringiensis and an E. coli recombinant have been determined. In B. thuringiensis var. israelensis, transcription initiates from a single start point, and gene-specific transcripts are not observed before stage II of sporulation. This is the stage at which δ-endotoxin antigen is first detected, indicating that control of expression is primarily at the transcriptional level for this protein. Analysis of gene-specific transcription in E. coli indicates that at least three start points are utilized in this organism. Interestingly, the highest level of δ-endotoxin mRNA is seen during mid-exponential growth of E. coli and the level appears to decrease as the cells enter the stationary phase of growth.

0022-2836
1-11
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199

Ward, E. S. and Ellar, D. J. (1986) Bacillus thuringiensis var. israelensis δ-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli. Journal of Molecular Biology, 191 (1), 1-11. (doi:10.1016/0022-2836(86)90417-1).

Record type: Article

Abstract

The nucleotide sequence of a 1408 base-pair DNA fragment encoding the insecticidal 27,340 Mr δ-endotoxin of Bacillus thuringiensis var. israelensis has been determined by analysis of a recombinant plasmid from Escherichia coli. The hydropathy plot of the protein shows it to be highly hydrophobic, consistent with a postulated cytolytic mechanism of action for the toxin. In addition, the δ-endotoxin transcriptional start points that are used in B. thuringiensis and an E. coli recombinant have been determined. In B. thuringiensis var. israelensis, transcription initiates from a single start point, and gene-specific transcripts are not observed before stage II of sporulation. This is the stage at which δ-endotoxin antigen is first detected, indicating that control of expression is primarily at the transcriptional level for this protein. Analysis of gene-specific transcription in E. coli indicates that at least three start points are utilized in this organism. Interestingly, the highest level of δ-endotoxin mRNA is seen during mid-exponential growth of E. coli and the level appears to decrease as the cells enter the stationary phase of growth.

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Published date: 5 September 1986

Identifiers

Local EPrints ID: 425247
URI: https://eprints.soton.ac.uk/id/eprint/425247
ISSN: 0022-2836
PURE UUID: 111c6b0b-0848-47f7-9315-6cc62d7067eb
ORCID for E. S. Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 12 Oct 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: E. S. Ward ORCID iD
Author: D. J. Ellar

University divisions

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