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Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai ICI toxic to both lepidoptera and diptera

Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai ICI toxic to both lepidoptera and diptera
Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai ICI toxic to both lepidoptera and diptera

Bacillus thuringiensis var. aizawai ICI synthesises an insecticidal protein δ-endotoxin (130-135 kDa) that is toxic to both lepidopteran and dipteran larvae, and cross-reacts immunologically with certain monospecific lepidopteran toxins. A 166-kb plasmid from this bacterium was found to hybridise with an intragenic probe derived from the cloned B. thuringiensis var. sotto lepidopteran-specific δ-endotoxin gene. A strongly hybridising 5.2-kb Sst I fragment from var. aizawai plasmid DNA was cloned in pUC18. After subcloning of this DNA in Escherichia coli, recombinants were obtained that synthesised large amounts of a 130-135-kDa protein. The protein was deposited in the cytoplasm as microscopically visible inclusion bodies and lysates of these cells were found to be toxic to both lepidopteran and dipteran larvae by comparison with controls. The structural basis for the dual specificity of this var. aizawai toxin is now amenable to further study.

Escherichia coli, gene probe, P1 toxins, plasmid vector, Recombinant DNA
0378-1119
285-290
Haider, M. Z.
9b563a47-c78b-4a8a-b987-e0dc14a19c80
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199
Haider, M. Z.
9b563a47-c78b-4a8a-b987-e0dc14a19c80
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ellar, D. J.
92e52b64-6600-401e-a3d0-b15cfff77199

Haider, M. Z., Ward, E. S. and Ellar, D. J. (1987) Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai ICI toxic to both lepidoptera and diptera. Gene, 52 (2-3), 285-290. (doi:10.1016/0378-1119(87)90055-2).

Record type: Article

Abstract

Bacillus thuringiensis var. aizawai ICI synthesises an insecticidal protein δ-endotoxin (130-135 kDa) that is toxic to both lepidopteran and dipteran larvae, and cross-reacts immunologically with certain monospecific lepidopteran toxins. A 166-kb plasmid from this bacterium was found to hybridise with an intragenic probe derived from the cloned B. thuringiensis var. sotto lepidopteran-specific δ-endotoxin gene. A strongly hybridising 5.2-kb Sst I fragment from var. aizawai plasmid DNA was cloned in pUC18. After subcloning of this DNA in Escherichia coli, recombinants were obtained that synthesised large amounts of a 130-135-kDa protein. The protein was deposited in the cytoplasm as microscopically visible inclusion bodies and lysates of these cells were found to be toxic to both lepidopteran and dipteran larvae by comparison with controls. The structural basis for the dual specificity of this var. aizawai toxin is now amenable to further study.

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More information

Accepted/In Press date: 19 December 1986
Published date: 1987
Keywords: Escherichia coli, gene probe, P1 toxins, plasmid vector, Recombinant DNA

Identifiers

Local EPrints ID: 425248
URI: https://eprints.soton.ac.uk/id/eprint/425248
ISSN: 0378-1119
PURE UUID: d5bc5d68-5e6f-451b-b0bd-42b082ca3bed
ORCID for E. S. Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 12 Oct 2018 16:30
Last modified: 13 Oct 2018 00:20

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Contributors

Author: M. Z. Haider
Author: E. S. Ward ORCID iD
Author: D. J. Ellar

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