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Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme

Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme
Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme

The associated heavy (VH) and light (VL) chain variable domains (FV) of the monoclonal anti-lysozyme antibody D1.3, secreted from Escherichia coli, have been crystallized in their antigen-bound and free forms. FvD1.3 gives tetragonal crystals, space group P41212 (or P43212), with a = 90·6 Å, c = 56·4 Å. The FvD1.3-lysozyme complex crystallizes in space group C2, with a = 129·2 Å, b = 60·8 Å, c = 56·9 Å and β = 119·3°. The crystals contain one molecule of Fv or of the Fv-lysozyme complex in their asymmetric units and diffract X-rays to high resolution, making them suitable for X-ray crystallographic studies.

0022-2836
617-619
Boulot, Ginette
808bb78c-ad10-4ec7-b656-25252342107d
Eiselé, Jean Luc
b2cf6935-003f-4a77-a336-b9808bc181ab
Bentley, Graham A.
b04e9112-0f57-4cda-85c5-aedff3b5ff1c
Bhat, T. Narayana
cbb00d2b-92b8-44de-89e1-65f933f1c7fc
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Winter, Greg
72106f71-2931-453a-861d-27625707b8fe
Poljak, Roberto J.
54d15fd7-cf40-42fd-96a2-c6f03159865b
Boulot, Ginette
808bb78c-ad10-4ec7-b656-25252342107d
Eiselé, Jean Luc
b2cf6935-003f-4a77-a336-b9808bc181ab
Bentley, Graham A.
b04e9112-0f57-4cda-85c5-aedff3b5ff1c
Bhat, T. Narayana
cbb00d2b-92b8-44de-89e1-65f933f1c7fc
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Winter, Greg
72106f71-2931-453a-861d-27625707b8fe
Poljak, Roberto J.
54d15fd7-cf40-42fd-96a2-c6f03159865b

Boulot, Ginette, Eiselé, Jean Luc, Bentley, Graham A., Bhat, T. Narayana, Ward, E. Sally, Winter, Greg and Poljak, Roberto J. (1990) Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme. Journal of Molecular Biology, 213 (4), 617-619. (doi:10.1016/S0022-2836(05)80248-7).

Record type: Article

Abstract

The associated heavy (VH) and light (VL) chain variable domains (FV) of the monoclonal anti-lysozyme antibody D1.3, secreted from Escherichia coli, have been crystallized in their antigen-bound and free forms. FvD1.3 gives tetragonal crystals, space group P41212 (or P43212), with a = 90·6 Å, c = 56·4 Å. The FvD1.3-lysozyme complex crystallizes in space group C2, with a = 129·2 Å, b = 60·8 Å, c = 56·9 Å and β = 119·3°. The crystals contain one molecule of Fv or of the Fv-lysozyme complex in their asymmetric units and diffract X-rays to high resolution, making them suitable for X-ray crystallographic studies.

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Accepted/In Press date: 12 February 1990
Published date: 20 June 1990

Identifiers

Local EPrints ID: 425255
URI: https://eprints.soton.ac.uk/id/eprint/425255
ISSN: 0022-2836
PURE UUID: 64f4d862-4137-4ac3-9101-e1dc542b34fb
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 12 Oct 2018 16:30
Last modified: 14 Mar 2019 01:21

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Contributors

Author: Ginette Boulot
Author: Jean Luc Eiselé
Author: Graham A. Bentley
Author: T. Narayana Bhat
Author: E. Sally Ward ORCID iD
Author: Greg Winter
Author: Roberto J. Poljak

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