Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme

Boulot, Ginette, Eiselé, Jean Luc, Bentley, Graham A., Bhat, T. Narayana, Ward, E. Sally, Winter, Greg and Poljak, Roberto J. (1990) Crystallization and preliminary X-ray diffraction study of the bacterially expressed Fv from the monoclonal anti-lysozyme antibody D1.3 and of its complex with the antigen, lysozyme. Journal of Molecular Biology, 213 (4), 617-619. (doi:10.1016/S0022-2836(05)80248-7).

Abstract

The associated heavy (V_H) and light (V_L) chain variable domains (F_V) of the monoclonal anti-lysozyme antibody D1.3, secreted from Escherichia coli, have been crystallized in their antigen-bound and free forms. FvD1.3 gives tetragonal crystals, space group P4₁2₁2 (or P4₃2₁2), with a = 90·6 Å, c = 56·4 Å. The FvD1.3-lysozyme complex crystallizes in space group C2, with a = 129·2 Å, b = 60·8 Å, c = 56·9 Å and β = 119·3°. The crystals contain one molecule of Fv or of the Fv-lysozyme complex in their asymmetric units and diffract X-rays to high resolution, making them suitable for X-ray crystallographic studies.

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Contributors

Author: E. Sally Ward

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