Expression and secretion of T‐Cell receptor Vα and Vβ domains using Escherichia coli as a host
Expression and secretion of T‐Cell receptor Vα and Vβ domains using Escherichia coli as a host
An expression system for the production of recombinant T‐cell receptor (TCR) variable domains would, inter alia, allow structural studies lo be carried out and provide protein for the generation of anti‐clonotypic antibodies, in this report the Vα and Vβ domain genes have been isolated from a T‐cell hybridoma which is associated with the pathogenesis of experimental allergic encephalomyelitis (EAE) In the H‐2 mouse. These have been expressed as secreted domains in Escherichia coli, using secretion vectors previously used for the production of immunoglobulin fragments. Both Vα and Vβ domains are secreted in milligram quantities into the culture supernatant, although the levels of the Vα domain are about 10‐20 Ibid higher than those of the Vβ domain. This expression system offers a rapid route for the production of recombinant TCRs in soluble form.
215-220
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
August 1991
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ward, E. S.
(1991)
Expression and secretion of T‐Cell receptor Vα and Vβ domains using Escherichia coli as a host.
Scandinavian Journal of Immunology, 34 (2), .
(doi:10.1111/j.1365-3083.1991.tb01539.x).
Abstract
An expression system for the production of recombinant T‐cell receptor (TCR) variable domains would, inter alia, allow structural studies lo be carried out and provide protein for the generation of anti‐clonotypic antibodies, in this report the Vα and Vβ domain genes have been isolated from a T‐cell hybridoma which is associated with the pathogenesis of experimental allergic encephalomyelitis (EAE) In the H‐2 mouse. These have been expressed as secreted domains in Escherichia coli, using secretion vectors previously used for the production of immunoglobulin fragments. Both Vα and Vβ domains are secreted in milligram quantities into the culture supernatant, although the levels of the Vα domain are about 10‐20 Ibid higher than those of the Vβ domain. This expression system offers a rapid route for the production of recombinant TCRs in soluble form.
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Published date: August 1991
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Local EPrints ID: 425256
URI: http://eprints.soton.ac.uk/id/eprint/425256
ISSN: 0300-9475
PURE UUID: 64f6aef3-9b31-4225-8fd8-0db4f32e1bc7
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Date deposited: 12 Oct 2018 16:30
Last modified: 16 Mar 2024 04:37
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