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Secretion of T cell receptor fragments from recombinant Escherichia coli cells

Secretion of T cell receptor fragments from recombinant Escherichia coli cells
Secretion of T cell receptor fragments from recombinant Escherichia coli cells

This study describes the secretion and purification of T cell receptor (TCR) Vα, Vβ domains and single chain Vα-Vβ fragments (scTCRs) from recombinant Escherichia coli cells. The TCR Vα and Vβ genes are derived from a T cell hybridoma that is associated with disease pathogenesis in murine experimental allergic encephalomyelitis (EAE). Circular dichroism (c.d.) analyses of the single domains and the scTCR indicate that they are folded into β-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted TCR fragments can be purified in milligram quantities, and could therefore be used in high-resolution structural studies, in immunization to generate anti-clonotypic antibodies or in vaccination.

circular dichroism, E. coli secretion, murine EAE, T cell receptor
0022-2836
885-890
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc
Ward, E. Sally
b31c0877-8abe-485f-b800-244a9d3cd6cc

Ward, E. Sally (1992) Secretion of T cell receptor fragments from recombinant Escherichia coli cells. Journal of Molecular Biology, 224 (4), 885-890. (doi:10.1016/0022-2836(92)90455-S).

Record type: Article

Abstract

This study describes the secretion and purification of T cell receptor (TCR) Vα, Vβ domains and single chain Vα-Vβ fragments (scTCRs) from recombinant Escherichia coli cells. The TCR Vα and Vβ genes are derived from a T cell hybridoma that is associated with disease pathogenesis in murine experimental allergic encephalomyelitis (EAE). Circular dichroism (c.d.) analyses of the single domains and the scTCR indicate that they are folded into β-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted TCR fragments can be purified in milligram quantities, and could therefore be used in high-resolution structural studies, in immunization to generate anti-clonotypic antibodies or in vaccination.

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More information

Accepted/In Press date: 20 December 1991
Published date: 20 April 1992
Keywords: circular dichroism, E. coli secretion, murine EAE, T cell receptor

Identifiers

Local EPrints ID: 425258
URI: https://eprints.soton.ac.uk/id/eprint/425258
ISSN: 0022-2836
PURE UUID: 3cd24bc7-563d-451a-add4-4a5e4d8c48b6
ORCID for E. Sally Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 12 Oct 2018 16:30
Last modified: 14 Mar 2019 01:21

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