Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi mmol-1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.
Deuterium, Isotope exchange, Proteins, Solid state hydrogenation, Tritium
179-185
Filikov, A. V.
aa3bdb31-b6df-4d64-a035-aacf94f7b4b5
Jones, J. R.
bb49db75-bb29-49be-818c-20880486c492
Myasoedov, N. F.
517b89ce-962c-4b56-b3eb-1165f4b12421
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
1994
Filikov, A. V.
aa3bdb31-b6df-4d64-a035-aacf94f7b4b5
Jones, J. R.
bb49db75-bb29-49be-818c-20880486c492
Myasoedov, N. F.
517b89ce-962c-4b56-b3eb-1165f4b12421
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Filikov, A. V., Jones, J. R., Myasoedov, N. F. and Ward, E. S.
(1994)
Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme.
Journal of Labelled Compounds and Radiopharmaceuticals, 35, .
Abstract
Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi mmol-1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.
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Published date: 1994
Keywords:
Deuterium, Isotope exchange, Proteins, Solid state hydrogenation, Tritium
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Local EPrints ID: 425266
URI: http://eprints.soton.ac.uk/id/eprint/425266
ISSN: 0362-4803
PURE UUID: 5c6eaec5-0dd0-4216-ada7-a4701eeb9d0f
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Date deposited: 12 Oct 2018 16:30
Last modified: 28 Feb 2024 03:05
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Contributors
Author:
A. V. Filikov
Author:
J. R. Jones
Author:
N. F. Myasoedov
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