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Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme

Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme

Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi mmol-1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.

Deuterium, Isotope exchange, Proteins, Solid state hydrogenation, Tritium
0362-4803
179-185
Filikov, A. V.
aa3bdb31-b6df-4d64-a035-aacf94f7b4b5
Jones, J. R.
bb49db75-bb29-49be-818c-20880486c492
Myasoedov, N. F.
517b89ce-962c-4b56-b3eb-1165f4b12421
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Filikov, A. V.
aa3bdb31-b6df-4d64-a035-aacf94f7b4b5
Jones, J. R.
bb49db75-bb29-49be-818c-20880486c492
Myasoedov, N. F.
517b89ce-962c-4b56-b3eb-1165f4b12421
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc

Filikov, A. V., Jones, J. R., Myasoedov, N. F. and Ward, E. S. (1994) Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme. Journal of Labelled Compounds and Radiopharmaceuticals, 35, 179-185.

Record type: Article

Abstract

Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi mmol-1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.

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More information

Published date: 1994
Keywords: Deuterium, Isotope exchange, Proteins, Solid state hydrogenation, Tritium

Identifiers

Local EPrints ID: 425266
URI: https://eprints.soton.ac.uk/id/eprint/425266
ISSN: 0362-4803
PURE UUID: 5c6eaec5-0dd0-4216-ada7-a4701eeb9d0f
ORCID for E. S. Ward: ORCID iD orcid.org/0000-0003-3232-7238

Catalogue record

Date deposited: 12 Oct 2018 16:30
Last modified: 22 May 2019 00:21

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Contributors

Author: A. V. Filikov
Author: J. R. Jones
Author: N. F. Myasoedov
Author: E. S. Ward ORCID iD

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