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Antimicrobial activity of the manganese photoactivated carbon monoxide-releasing molecule [Mn(CO) 3 (tpa-κ 3 N )] + against a pathogenic Escherichia coli that causes urinary infections

Antimicrobial activity of the manganese photoactivated carbon monoxide-releasing molecule [Mn(CO) 3 (tpa-κ 3 N )] + against a pathogenic Escherichia coli that causes urinary infections
Antimicrobial activity of the manganese photoactivated carbon monoxide-releasing molecule [Mn(CO) 3 (tpa-κ 3 N )] + against a pathogenic Escherichia coli that causes urinary infections
Abstract Aims: We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)3(tpa-κ3N)]+) against an antibiotic-resistant uropathogenic strain (EC958) of Escherichia coli. Results: Activated PhotoCORM inhibits growth and decreases viability of E. coli EC958, but non-illuminated carbon monoxide-releasing molecule (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking the effect of dissolved CO gas. CO from the PhotoCORM binds to intracellular targets, namely respiratory oxidases in strain EC958 and a bacterial globin heterologously expressed in strain K-12. However, unlike previously characterized CORMs, the PhotoCORM is not significantly accumulated in cells, as deduced from the cellular manganese content. Activated PhotoCORM reacts avidly with hydrogen peroxide producing hydroxyl radicals; the observed peroxide-enhanced toxicity of the PhotoCORM is ameliorated b...
1523-0864
765-780
Tinajero-Trejo, Mariana
b192a249-82e9-48b9-bf8e-c9f0c451587a
Rana, Namrata
d0817708-17a1-4bf5-af21-08348dbcbd1c
Nagel, Christoph
e4d1042a-3c7e-4ae4-835c-61abf4690492
Jesse, Helen E.
c4f50e4c-ba5f-4c93-80d2-336f6ebf4d4e
Smith, Thomas W.
890d7d44-ab77-46cd-9c24-803936f6ac99
Wareham, Lauren K.
5e760311-680f-4355-944e-3cbc7e6354a1
Hippler, Michael
14b18bbd-6ebd-48c1-b1f0-c8161a18e40f
Schatzschneider, Ulrich
c39bcf5f-08c6-408c-b075-dfe48649de96
Poole, Robert K.
5f1f3b79-cf45-4ae6-89cd-e4259c03ae56
Tinajero-Trejo, Mariana
b192a249-82e9-48b9-bf8e-c9f0c451587a
Rana, Namrata
d0817708-17a1-4bf5-af21-08348dbcbd1c
Nagel, Christoph
e4d1042a-3c7e-4ae4-835c-61abf4690492
Jesse, Helen E.
c4f50e4c-ba5f-4c93-80d2-336f6ebf4d4e
Smith, Thomas W.
890d7d44-ab77-46cd-9c24-803936f6ac99
Wareham, Lauren K.
5e760311-680f-4355-944e-3cbc7e6354a1
Hippler, Michael
14b18bbd-6ebd-48c1-b1f0-c8161a18e40f
Schatzschneider, Ulrich
c39bcf5f-08c6-408c-b075-dfe48649de96
Poole, Robert K.
5f1f3b79-cf45-4ae6-89cd-e4259c03ae56

Tinajero-Trejo, Mariana, Rana, Namrata, Nagel, Christoph, Jesse, Helen E., Smith, Thomas W., Wareham, Lauren K., Hippler, Michael, Schatzschneider, Ulrich and Poole, Robert K. (2016) Antimicrobial activity of the manganese photoactivated carbon monoxide-releasing molecule [Mn(CO) 3 (tpa-κ 3 N )] + against a pathogenic Escherichia coli that causes urinary infections. Antioxidants & Redox Signaling, 24 (14), 765-780. (doi:10.1089/ars.2015.6484).

Record type: Article

Abstract

Abstract Aims: We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)3(tpa-κ3N)]+) against an antibiotic-resistant uropathogenic strain (EC958) of Escherichia coli. Results: Activated PhotoCORM inhibits growth and decreases viability of E. coli EC958, but non-illuminated carbon monoxide-releasing molecule (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking the effect of dissolved CO gas. CO from the PhotoCORM binds to intracellular targets, namely respiratory oxidases in strain EC958 and a bacterial globin heterologously expressed in strain K-12. However, unlike previously characterized CORMs, the PhotoCORM is not significantly accumulated in cells, as deduced from the cellular manganese content. Activated PhotoCORM reacts avidly with hydrogen peroxide producing hydroxyl radicals; the observed peroxide-enhanced toxicity of the PhotoCORM is ameliorated b...

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e-pub ahead of print date: 4 February 2016
Published date: 10 May 2016

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Local EPrints ID: 425313
URI: http://eprints.soton.ac.uk/id/eprint/425313
ISSN: 1523-0864
PURE UUID: e3d4f1a2-20ad-4533-99d7-72a06dde2974

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Date deposited: 12 Oct 2018 16:30
Last modified: 09 Nov 2021 14:17

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Contributors

Author: Mariana Tinajero-Trejo
Author: Namrata Rana
Author: Christoph Nagel
Author: Helen E. Jesse
Author: Thomas W. Smith
Author: Lauren K. Wareham
Author: Michael Hippler
Author: Ulrich Schatzschneider
Author: Robert K. Poole

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