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Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species

Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species
Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species
H. haemolyticus is often misidentified as NTHi due to their close phylogenetic relationship. Differentiating between the two is important for correct identification and appropriate treatment of infective organism and to ensure any role of H. haemolyticus in disease is not being overlooked. Speciation however is not completely reliable by culture and PCR methods due to the loss of haemolysis by H. haemolyticus and the heterogeneity of NTHi. Haemophilus isolates from COPD as part of the AERIS study (ClinicalTrials NCT01360398) were speciated by analysing sequence data for the presence of molecular markers. Further investigation into the genomic relationship was carried out using average nucleotide identity and phylogeny of allelic and genome alignments. Only 6.3% were identified as H. haemolyticus. Multiple in silico methods were able to distinguish H. haemolyticus from NTHi. However, no single gene target was found to be 100% accurate. A group of omp2 negative NTHi were observed to be phylogenetically divergent from H. haemolyticus and remaining NTHi. The presence of an atypical group from a geographically and disease limited set of isolates supports the theory that the heterogeneity of NTHi may provide a genetic continuum between NTHi and H. haemolyticus.
2045-2322
Osman, Karen
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Jefferies, Johanna M.C.
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Woelk, Christopher H
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Devos, Nathalie
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Pascal, Thierry G.
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Mortier, Marie-Cécile
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Devaster, Jeanne-Marie
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Wilkinson, T.M.A.
1583ef8c-8ca9-4d46-ae60-0b3556090d8f
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Clarke, Stuart
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AERIS Study Group
Osman, Karen
10ca3ab1-b308-44f3-9dcd-75a522220bf0
Jefferies, Johanna M.C.
942356b7-2070-49ad-9376-cde03c5fe13d
Woelk, Christopher H
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Devos, Nathalie
376b1648-5ac2-4c5e-92c7-9cf228fda87c
Pascal, Thierry G.
dd956192-fbab-4de3-bbac-9bca6b304700
Mortier, Marie-Cécile
976603db-f503-48a8-b144-f63bd5ed142b
Devaster, Jeanne-Marie
36195bcf-0cae-40cc-8ee2-d389a4a65a13
Wilkinson, T.M.A.
1583ef8c-8ca9-4d46-ae60-0b3556090d8f
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Clarke, Stuart
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Osman, Karen, Jefferies, Johanna M.C., Woelk, Christopher H, Devos, Nathalie, Pascal, Thierry G., Mortier, Marie-Cécile, Devaster, Jeanne-Marie, Wilkinson, T.M.A., Cleary, David and Clarke, Stuart , AERIS Study Group (2018) Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species. Scientific Reports, 8 (1). (doi:10.1038/s41598-018-32973-3).

Record type: Article

Abstract

H. haemolyticus is often misidentified as NTHi due to their close phylogenetic relationship. Differentiating between the two is important for correct identification and appropriate treatment of infective organism and to ensure any role of H. haemolyticus in disease is not being overlooked. Speciation however is not completely reliable by culture and PCR methods due to the loss of haemolysis by H. haemolyticus and the heterogeneity of NTHi. Haemophilus isolates from COPD as part of the AERIS study (ClinicalTrials NCT01360398) were speciated by analysing sequence data for the presence of molecular markers. Further investigation into the genomic relationship was carried out using average nucleotide identity and phylogeny of allelic and genome alignments. Only 6.3% were identified as H. haemolyticus. Multiple in silico methods were able to distinguish H. haemolyticus from NTHi. However, no single gene target was found to be 100% accurate. A group of omp2 negative NTHi were observed to be phylogenetically divergent from H. haemolyticus and remaining NTHi. The presence of an atypical group from a geographically and disease limited set of isolates supports the theory that the heterogeneity of NTHi may provide a genetic continuum between NTHi and H. haemolyticus.

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Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species - Accepted Manuscript
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Accepted/In Press date: 19 September 2018
e-pub ahead of print date: 3 October 2018
Published date: 3 October 2018

Identifiers

Local EPrints ID: 425373
URI: https://eprints.soton.ac.uk/id/eprint/425373
ISSN: 2045-2322
PURE UUID: a4556511-4a88-438a-9242-d23d00e83131
ORCID for David Cleary: ORCID iD orcid.org/0000-0003-4533-0700
ORCID for Stuart Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 16 Oct 2018 16:30
Last modified: 12 Nov 2019 01:46

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Contributors

Author: Karen Osman
Author: Johanna M.C. Jefferies
Author: Nathalie Devos
Author: Thierry G. Pascal
Author: Marie-Cécile Mortier
Author: Jeanne-Marie Devaster
Author: T.M.A. Wilkinson
Author: David Cleary ORCID iD
Author: Stuart Clarke ORCID iD

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