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Vaccination with a recombinant Vα domain of a TCR prevents the development of collagen-induced arthritis

Vaccination with a recombinant Vα domain of a TCR prevents the development of collagen-induced arthritis
Vaccination with a recombinant Vα domain of a TCR prevents the development of collagen-induced arthritis

A recombinant TCR domain, derived from a T cell hybridoma that recognizes an immunodominant type II collagen epitope, was used to vaccinate against collagen-induced arthritis in DBA/1 (H-2(q)) mice. The recombinant TCR domain comprises VA11.1-JA17 gene segments and is representative of the Vα domains expressed by oligoclonal T cells in this disease model. Vaccination of mice 28 days before type II collagen (CII) immunization with this Vα11.1 domain resulted in a significantly decreased incidence of arthritis in DBA/1 mice, in contrast to vaccination with a Vα4-Jα40 domain derived from an encephalitogenic T cell hybridoma specific for MBP. Disease blockade is accompanied by a reduction in T and B cell responses to both the immunogen bovine CII and the autoantigen murine CII. Vα4 and Vα11.1 domains were found to be highly immunogenic in DBA/1 mice, inducing both T cell proliferation and the production of Vα specific Abs, indicating that the vaccination effect of Vα11.1 is specific. This is the first report of Vα- directed immunotherapy in an autoimmune disease model and demonstrates the potential use of recombinant TCR vaccines in the treatment of autoimmune diseases that involve oligoclonal autoreactive T cells.

0022-1767
4504-4511
Rosloniec, E. F.
2aee8d14-4548-4f55-9656-4c7badb3e928
Brand, D. D.
9f5ac8dc-d2c4-41b3-bd5f-8298115c059d
Whittington, K. B.
9767e29c-ab0c-485b-a428-01f2a84cd297
Stuart, J. M.
06b8de08-2aef-4e9d-bca1-6831f07315ab
Ciubotaru, M.
bc4ca202-4bea-41ad-86ca-a0fd8527b48f
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc
Rosloniec, E. F.
2aee8d14-4548-4f55-9656-4c7badb3e928
Brand, D. D.
9f5ac8dc-d2c4-41b3-bd5f-8298115c059d
Whittington, K. B.
9767e29c-ab0c-485b-a428-01f2a84cd297
Stuart, J. M.
06b8de08-2aef-4e9d-bca1-6831f07315ab
Ciubotaru, M.
bc4ca202-4bea-41ad-86ca-a0fd8527b48f
Ward, E. S.
b31c0877-8abe-485f-b800-244a9d3cd6cc

Rosloniec, E. F., Brand, D. D., Whittington, K. B., Stuart, J. M., Ciubotaru, M. and Ward, E. S. (1995) Vaccination with a recombinant Vα domain of a TCR prevents the development of collagen-induced arthritis. Journal of Immunology, 155 (9), 4504-4511.

Record type: Article

Abstract

A recombinant TCR domain, derived from a T cell hybridoma that recognizes an immunodominant type II collagen epitope, was used to vaccinate against collagen-induced arthritis in DBA/1 (H-2(q)) mice. The recombinant TCR domain comprises VA11.1-JA17 gene segments and is representative of the Vα domains expressed by oligoclonal T cells in this disease model. Vaccination of mice 28 days before type II collagen (CII) immunization with this Vα11.1 domain resulted in a significantly decreased incidence of arthritis in DBA/1 mice, in contrast to vaccination with a Vα4-Jα40 domain derived from an encephalitogenic T cell hybridoma specific for MBP. Disease blockade is accompanied by a reduction in T and B cell responses to both the immunogen bovine CII and the autoantigen murine CII. Vα4 and Vα11.1 domains were found to be highly immunogenic in DBA/1 mice, inducing both T cell proliferation and the production of Vα specific Abs, indicating that the vaccination effect of Vα11.1 is specific. This is the first report of Vα- directed immunotherapy in an autoimmune disease model and demonstrates the potential use of recombinant TCR vaccines in the treatment of autoimmune diseases that involve oligoclonal autoreactive T cells.

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Published date: 1995

Identifiers

Local EPrints ID: 425407
URI: http://eprints.soton.ac.uk/id/eprint/425407
ISSN: 0022-1767
PURE UUID: 2db2cc47-aaac-4976-a73d-2e665cec710a
ORCID for E. S. Ward: ORCID iD orcid.org/0000-0003-3232-7238

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Date deposited: 18 Oct 2018 16:30
Last modified: 07 Oct 2020 02:22

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Contributors

Author: E. F. Rosloniec
Author: D. D. Brand
Author: K. B. Whittington
Author: J. M. Stuart
Author: M. Ciubotaru
Author: E. S. Ward ORCID iD

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