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NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level

NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level
NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level
Understanding the molecular mechanisms governing the uptake and accumulation of Zn in the leaves of tobacco plants exposed to high Zn concentrations is important from the perspective of phytoremediation of metal contaminated soil. This study identifies a new candidate gene, NtZIP11, which may contribute to Zn accumulation in tobacco leaves. NtZIP11 encodes a protein of 346 amino acids, with characteristic conserved sequences of the ZIP family of proteins. Phylogenetic analysis shows that NtZIP11 forms a distinct clade with other ZIP11 proteins. Transient expression of GFP-tagged ZIP11 in the abaxial epidermis of tobacco leaves demonstrates localization at the plasma membrane. Yeast complementation tests and growth assays indicate that NtZIP11 is involved in Zn but not Fe, Mn and Cd uptake. NtZIP11 complements the zrt1zrt2 mutant deficient in Zn uptake, but not the fet3fet4 mutant deficient in Fe uptake. Nor does it modify the sensitivity of wild-type yeast, DY1457, to increasing concentrations of Fe, Mn and Cd. NtZIP11 is expressed in both roots and leaves, with transcript abundance increasing with age. Noteworthy, the expression level of NtZIP11 is not modulated by Zn-deficiency but is highly upregulated especially in the older leaves by high Zn concentrations (50 and 200 µM). Our data indicate that the primary role for NtZIP11 is in the uptake of Zn by the cells of tobacco leaves specifically when experiencing high Zn concentrations. It also likely contributes to maintaining a basal supply of Zn to cells at the level of the whole plant. Thus the present study contributes to broadening our understanding of Zn homeostasis mechanisms in tobacco, and clarifying the role of ZIP11 genes.
0098-8472
69-78
Kozak, Katarzyna
d9372f54-281d-4d78-9e3a-c72d5e61a97c
Papierniak, Anna
7366e0b3-9da3-4336-9736-db8cc5fc9dfc
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Kendziorek, Maria
dd1ce2ec-4b2f-4dae-b4c5-437901fc7c33
Palusińska, Małgorzata
9a7fcac0-2ac6-4cf3-8c2e-3d41e6f42185
Williams, Lorraine
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b
Kozak, Katarzyna
d9372f54-281d-4d78-9e3a-c72d5e61a97c
Papierniak, Anna
7366e0b3-9da3-4336-9736-db8cc5fc9dfc
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Kendziorek, Maria
dd1ce2ec-4b2f-4dae-b4c5-437901fc7c33
Palusińska, Małgorzata
9a7fcac0-2ac6-4cf3-8c2e-3d41e6f42185
Williams, Lorraine
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b

Kozak, Katarzyna, Papierniak, Anna, Barabasz, Anna, Kendziorek, Maria, Palusińska, Małgorzata, Williams, Lorraine and Antosiewicz, Danuta Maria (2019) NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level. Environmental and Experimental Botany, 157, 69-78. (doi:10.1016/j.envexpbot.2018.09.020).

Record type: Article

Abstract

Understanding the molecular mechanisms governing the uptake and accumulation of Zn in the leaves of tobacco plants exposed to high Zn concentrations is important from the perspective of phytoremediation of metal contaminated soil. This study identifies a new candidate gene, NtZIP11, which may contribute to Zn accumulation in tobacco leaves. NtZIP11 encodes a protein of 346 amino acids, with characteristic conserved sequences of the ZIP family of proteins. Phylogenetic analysis shows that NtZIP11 forms a distinct clade with other ZIP11 proteins. Transient expression of GFP-tagged ZIP11 in the abaxial epidermis of tobacco leaves demonstrates localization at the plasma membrane. Yeast complementation tests and growth assays indicate that NtZIP11 is involved in Zn but not Fe, Mn and Cd uptake. NtZIP11 complements the zrt1zrt2 mutant deficient in Zn uptake, but not the fet3fet4 mutant deficient in Fe uptake. Nor does it modify the sensitivity of wild-type yeast, DY1457, to increasing concentrations of Fe, Mn and Cd. NtZIP11 is expressed in both roots and leaves, with transcript abundance increasing with age. Noteworthy, the expression level of NtZIP11 is not modulated by Zn-deficiency but is highly upregulated especially in the older leaves by high Zn concentrations (50 and 200 µM). Our data indicate that the primary role for NtZIP11 is in the uptake of Zn by the cells of tobacco leaves specifically when experiencing high Zn concentrations. It also likely contributes to maintaining a basal supply of Zn to cells at the level of the whole plant. Thus the present study contributes to broadening our understanding of Zn homeostasis mechanisms in tobacco, and clarifying the role of ZIP11 genes.

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2018 Kozak NtZIP11 EEB accepted - Accepted Manuscript
Restricted to Repository staff only until 27 September 2019.
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More information

Accepted/In Press date: 24 September 2018
e-pub ahead of print date: 27 September 2018
Published date: January 2019

Identifiers

Local EPrints ID: 425458
URI: https://eprints.soton.ac.uk/id/eprint/425458
ISSN: 0098-8472
PURE UUID: 8c9a44c2-d3e4-491d-85eb-d58ce8d1d2ad

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Date deposited: 19 Oct 2018 16:30
Last modified: 13 Mar 2019 17:59

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