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Nitrate drawdown during a shelf sea spring bloom revealed using a novel microfluidic in situ chemical sensor deployed within an autonomous underwater glider

Nitrate drawdown during a shelf sea spring bloom revealed using a novel microfluidic in situ chemical sensor deployed within an autonomous underwater glider
Nitrate drawdown during a shelf sea spring bloom revealed using a novel microfluidic in situ chemical sensor deployed within an autonomous underwater glider

Here we describe, for the first time, the use of a miniaturized Lab-on-Chip (LoC) nutrient sensor deployed within an autonomous underwater vehicle (AUV; Kongsberg Seaglider) to collect high-resolution nitrate (nitrate + nitrite) data in a highly dynamic shelf sea environment. Seasonally stratified temperate shelf seas act as important carbon sinks, where primary production is controlled by the availability of nutrients such as nitrate. Spring phytoplankton blooms and sporadic mixing events can drastically modify the availability of nitrate on temporal scales from hours to days. Traditional sampling methods are unable to capture high frequency events that can be clearly observed using a wet-chemical microfluidic system deployed within a glider. We highlight firstly, an excellent agreement between the LoC and shipboard nitrate + nitrite measurements (r2 = 0.98 n = 11). Secondly, the LoC was able to observe a decrease in nitrate within the surface mixed layer from 5.74 μM (4th) to 1.42 μM (25th) during the onset of the spring bloom, whilst bottom layer concentrations remained constant (6.86 ± 0.16 μM), with an estimated analytical uncertaintly of <0.2 μM. Thirdly, the ability of an LoC sensor deployed within an AUV to accurately capture simultaneous biogeochemical and physical parameters at an enhanced resolution, on both spatial and temporal scales, improves our understanding of biogeochemical cycles within the dynamic temperate shelf sea environments.

Autonomous underwater vehicles, Microfluidics, Nutrients, Shelf seas
0304-4203
29-36
Vincent, Alexander G.
d99d8f6f-7273-478d-8f94-aa62773aa758
Pascal, Robin W.
4e77e1e1-289b-4156-9310-4ab3287300c4
Beaton, Alexander D.
02f38dc0-7db0-488c-af92-465275f9d584
Walk, John
17dfab03-5699-4f13-bf08-24c23ee11845
Hopkins, Joanne E.
d477d6d1-e91f-4ef7-9c56-50e3e7cbf7be
Woodward, E. Malcolm S.
3a11b7bf-b110-448a-b1ee-ae1ce7d0fef5
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f
Lohan, Maeve C.
6ca10597-2d0f-40e8-8e4f-7619dfac5088
Vincent, Alexander G.
d99d8f6f-7273-478d-8f94-aa62773aa758
Pascal, Robin W.
4e77e1e1-289b-4156-9310-4ab3287300c4
Beaton, Alexander D.
02f38dc0-7db0-488c-af92-465275f9d584
Walk, John
17dfab03-5699-4f13-bf08-24c23ee11845
Hopkins, Joanne E.
d477d6d1-e91f-4ef7-9c56-50e3e7cbf7be
Woodward, E. Malcolm S.
3a11b7bf-b110-448a-b1ee-ae1ce7d0fef5
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f
Lohan, Maeve C.
6ca10597-2d0f-40e8-8e4f-7619dfac5088

Vincent, Alexander G., Pascal, Robin W., Beaton, Alexander D., Walk, John, Hopkins, Joanne E., Woodward, E. Malcolm S., Mowlem, Matthew and Lohan, Maeve C. (2018) Nitrate drawdown during a shelf sea spring bloom revealed using a novel microfluidic in situ chemical sensor deployed within an autonomous underwater glider. Marine Chemistry, 205, 29-36. (doi:10.1016/j.marchem.2018.07.005).

Record type: Article

Abstract

Here we describe, for the first time, the use of a miniaturized Lab-on-Chip (LoC) nutrient sensor deployed within an autonomous underwater vehicle (AUV; Kongsberg Seaglider) to collect high-resolution nitrate (nitrate + nitrite) data in a highly dynamic shelf sea environment. Seasonally stratified temperate shelf seas act as important carbon sinks, where primary production is controlled by the availability of nutrients such as nitrate. Spring phytoplankton blooms and sporadic mixing events can drastically modify the availability of nitrate on temporal scales from hours to days. Traditional sampling methods are unable to capture high frequency events that can be clearly observed using a wet-chemical microfluidic system deployed within a glider. We highlight firstly, an excellent agreement between the LoC and shipboard nitrate + nitrite measurements (r2 = 0.98 n = 11). Secondly, the LoC was able to observe a decrease in nitrate within the surface mixed layer from 5.74 μM (4th) to 1.42 μM (25th) during the onset of the spring bloom, whilst bottom layer concentrations remained constant (6.86 ± 0.16 μM), with an estimated analytical uncertaintly of <0.2 μM. Thirdly, the ability of an LoC sensor deployed within an AUV to accurately capture simultaneous biogeochemical and physical parameters at an enhanced resolution, on both spatial and temporal scales, improves our understanding of biogeochemical cycles within the dynamic temperate shelf sea environments.

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More information

Accepted/In Press date: 14 July 2018
e-pub ahead of print date: 17 July 2018
Published date: 20 September 2018
Keywords: Autonomous underwater vehicles, Microfluidics, Nutrients, Shelf seas

Identifiers

Local EPrints ID: 425606
URI: http://eprints.soton.ac.uk/id/eprint/425606
ISSN: 0304-4203
PURE UUID: 384f0592-2686-4fec-a9b9-029c3472ae3d
ORCID for Maeve C. Lohan: ORCID iD orcid.org/0000-0002-5340-3108

Catalogue record

Date deposited: 25 Oct 2018 16:30
Last modified: 07 Oct 2020 02:02

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Contributors

Author: Alexander G. Vincent
Author: Robin W. Pascal
Author: Alexander D. Beaton
Author: John Walk
Author: Joanne E. Hopkins
Author: E. Malcolm S. Woodward
Author: Matthew Mowlem
Author: Maeve C. Lohan ORCID iD

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