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A marine biofilm flow cell for in situ screening marine fouling control coatings using optical coherence tomography

A marine biofilm flow cell for in situ screening marine fouling control coatings using optical coherence tomography
A marine biofilm flow cell for in situ screening marine fouling control coatings using optical coherence tomography
A novel fouling marine flow cell was designed and fitted with a clear plastic lid to allow real-time imaging of biofilms using optical coherence tomography (OCT). Marine biofilms were grown under controlled shear flow on coupons coated with 6 different biocidal antifouling coatings (SPC1, SPC2, SPC3, SPC4, CDP1 and CDP2, AkzoNobel) and one inert coating which contained no biocidal actives (NB-D) for 8 weeks. One set of coupons coated with NB was statically immersed in sea water during the same time period (NB-S). Biofilm removal was assessed by increasing the flow velocity while OCT simultaneously measured the biofilm cross-sectional area (CSA). The highest initial removal rates were observed for NB-S, NB-D, and SPC2. Percent biofilm cross-sectional area reduction (%CSAred) was higher on SPCs (>60%) compared to CDPs (<50%). SPCs had the highest percent reduction in biofilm surface area coverage (%SACred >60%) compared to the CDPs (<20 %). The marine biofilm flow cell combined with OCT can be used to screen for coating-specific differences in biofilm growth and removal in real time rather than traditional before and after surface area coverage measurements. Future testing will focus on how the biofilm-coatings interactions interact with biofilm mechanical and structural properties to produce drag.
biofilms, marine biofilms, flow cell, fouling, drag, OCT
0029-8018
321-328
Fabbri, Stefania
c93b6166-2117-48a9-9a88-b23a62c7b5da
Dennington, Simon
6a329a55-8c10-4515-8920-d8f40f302221
Price, Clayton
5593a52f-5781-4c27-ac3f-caf478d86640
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Longyear, Jennifer, Elise
fbc8ed1c-9fe4-45c3-bda4-7ca64551e5ce
Fabbri, Stefania
c93b6166-2117-48a9-9a88-b23a62c7b5da
Dennington, Simon
6a329a55-8c10-4515-8920-d8f40f302221
Price, Clayton
5593a52f-5781-4c27-ac3f-caf478d86640
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Longyear, Jennifer, Elise
fbc8ed1c-9fe4-45c3-bda4-7ca64551e5ce

Fabbri, Stefania, Dennington, Simon, Price, Clayton, Stoodley, Paul and Longyear, Jennifer, Elise (2018) A marine biofilm flow cell for in situ screening marine fouling control coatings using optical coherence tomography. Ocean Engineering, 170, 321-328. (doi:10.1016/j.oceaneng.2018.10.030).

Record type: Article

Abstract

A novel fouling marine flow cell was designed and fitted with a clear plastic lid to allow real-time imaging of biofilms using optical coherence tomography (OCT). Marine biofilms were grown under controlled shear flow on coupons coated with 6 different biocidal antifouling coatings (SPC1, SPC2, SPC3, SPC4, CDP1 and CDP2, AkzoNobel) and one inert coating which contained no biocidal actives (NB-D) for 8 weeks. One set of coupons coated with NB was statically immersed in sea water during the same time period (NB-S). Biofilm removal was assessed by increasing the flow velocity while OCT simultaneously measured the biofilm cross-sectional area (CSA). The highest initial removal rates were observed for NB-S, NB-D, and SPC2. Percent biofilm cross-sectional area reduction (%CSAred) was higher on SPCs (>60%) compared to CDPs (<50%). SPCs had the highest percent reduction in biofilm surface area coverage (%SACred >60%) compared to the CDPs (<20 %). The marine biofilm flow cell combined with OCT can be used to screen for coating-specific differences in biofilm growth and removal in real time rather than traditional before and after surface area coverage measurements. Future testing will focus on how the biofilm-coatings interactions interact with biofilm mechanical and structural properties to produce drag.

Text
OCT manuscript REVIEWED - Accepted Manuscript
Restricted to Repository staff only until 2 November 2020.
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More information

Accepted/In Press date: 21 October 2018
e-pub ahead of print date: 2 November 2018
Published date: 15 December 2018
Keywords: biofilms, marine biofilms, flow cell, fouling, drag, OCT

Identifiers

Local EPrints ID: 425645
URI: http://eprints.soton.ac.uk/id/eprint/425645
ISSN: 0029-8018
PURE UUID: cbc2f803-198b-4fbc-9e8f-d673d9d915b5
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 30 Oct 2018 17:30
Last modified: 17 Dec 2019 01:42

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