The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Wide-field multiphoton imaging through scattering media without correction

Wide-field multiphoton imaging through scattering media without correction
Wide-field multiphoton imaging through scattering media without correction
Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.
2375-2548
Escobet-Montalbàn, Adrià
ffa5c843-d87b-413a-bc82-cef2733e7870
Spesyvtsev, Roman
8c98e431-9792-4841-b3f7-4fa0de0c06c7
Chen, Mingzhou
79a02a48-4960-4670-af3e-4a0004364235
Saber, Wardiya Afshar
effbce55-9f04-4b89-bedb-0df42d7780ec
Andrews, Melissa R.
ae987a2f-878e-4ae3-a7a3-a7170712096c
Herrington, C. Simon
8fd9be08-96a4-4e40-ba2e-63d239640d7f
Mazilu, Michael
abc73a0d-8200-46f6-a2b9-47d82541c479
Dholakia, Kishan
c649b0a9-ab68-4571-b8c7-e84553d14174
Escobet-Montalbàn, Adrià
ffa5c843-d87b-413a-bc82-cef2733e7870
Spesyvtsev, Roman
8c98e431-9792-4841-b3f7-4fa0de0c06c7
Chen, Mingzhou
79a02a48-4960-4670-af3e-4a0004364235
Saber, Wardiya Afshar
effbce55-9f04-4b89-bedb-0df42d7780ec
Andrews, Melissa R.
ae987a2f-878e-4ae3-a7a3-a7170712096c
Herrington, C. Simon
8fd9be08-96a4-4e40-ba2e-63d239640d7f
Mazilu, Michael
abc73a0d-8200-46f6-a2b9-47d82541c479
Dholakia, Kishan
c649b0a9-ab68-4571-b8c7-e84553d14174

Escobet-Montalbàn, Adrià, Spesyvtsev, Roman, Chen, Mingzhou, Saber, Wardiya Afshar, Andrews, Melissa R., Herrington, C. Simon, Mazilu, Michael and Dholakia, Kishan (2018) Wide-field multiphoton imaging through scattering media without correction. Science Advances, 4 (10), [eaau1338]. (doi:10.1126/sciadv.aau1338).

Record type: Article

Abstract

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

Text
eaau1338.full - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 7 September 2018
e-pub ahead of print date: 12 October 2018
Published date: 12 October 2018

Identifiers

Local EPrints ID: 425648
URI: http://eprints.soton.ac.uk/id/eprint/425648
ISSN: 2375-2548
PURE UUID: 1a1cd476-d462-4a91-906f-b7a47fff8cb0
ORCID for Melissa R. Andrews: ORCID iD orcid.org/0000-0001-5960-5619

Catalogue record

Date deposited: 30 Oct 2018 17:30
Last modified: 26 Nov 2021 03:09

Export record

Altmetrics

Contributors

Author: Adrià Escobet-Montalbàn
Author: Roman Spesyvtsev
Author: Mingzhou Chen
Author: Wardiya Afshar Saber
Author: C. Simon Herrington
Author: Michael Mazilu
Author: Kishan Dholakia

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×