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Concentration-dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans

Concentration-dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans
Concentration-dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans
Background: neonicotinoids insecticides are under review due to emerging toxicity to non-target species. Interest has focused on biological pollinators whilst their effects on other organisms that are key contributors to the ecosystem remain largely unknown. To advance this we have tested the effects of representatives of three major classes of neonicotinoids, thiacloprid, clothianidin and nitenpyram on the free-living nematode Caenorhabditis elegans (C. elegans), as a representative of the Nematoda, an ecologically important phylum contributing to biomass.

Results: concentrations that are several-fold higher than those with effects against target species had limited impact on locomotor function. However, increased potency was observed in a mutant with a hyper-permeable cuticle which shows that drug access limits the effects of the neonicotinoids in C. elegans. Thiacloprid was most potent (EC50 714 µM). In addition, it selectively delayed larval development in wild-type worms at 1 mM.

Conclusions: C. elegans is less susceptible to neonicotinoids than target species of pest insect. We discuss an approach in which this defined low sensitivity may be exploited by heterologous expression of insect nicotinic acetylcholine receptors from both pest and beneficial insects, in transgenic C. elegans with increased cuticle permeability to provide a whole organism assay for species-dependent neonicotinoid effects.
1526-4998
Kudelska, Monika M.
5c14171a-f086-40ea-888a-f36035e8bc67
Holden-Dye, Linda
8032bf60-5db6-40cb-b71c-ddda9d212c8e
O'Connor, Vincent
2fe9031c-708c-4994-98e8-c2a33781d642
Doyle, Declan
f85f52c8-ce43-4f15-bd06-1df106f73b26
Kudelska, Monika M.
5c14171a-f086-40ea-888a-f36035e8bc67
Holden-Dye, Linda
8032bf60-5db6-40cb-b71c-ddda9d212c8e
O'Connor, Vincent
2fe9031c-708c-4994-98e8-c2a33781d642
Doyle, Declan
f85f52c8-ce43-4f15-bd06-1df106f73b26

Kudelska, Monika M., Holden-Dye, Linda, O'Connor, Vincent and Doyle, Declan (2017) Concentration-dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans. Pest Management Science, 73 (7). (doi:10.1002/ps.4564).

Record type: Article

Abstract

Background: neonicotinoids insecticides are under review due to emerging toxicity to non-target species. Interest has focused on biological pollinators whilst their effects on other organisms that are key contributors to the ecosystem remain largely unknown. To advance this we have tested the effects of representatives of three major classes of neonicotinoids, thiacloprid, clothianidin and nitenpyram on the free-living nematode Caenorhabditis elegans (C. elegans), as a representative of the Nematoda, an ecologically important phylum contributing to biomass.

Results: concentrations that are several-fold higher than those with effects against target species had limited impact on locomotor function. However, increased potency was observed in a mutant with a hyper-permeable cuticle which shows that drug access limits the effects of the neonicotinoids in C. elegans. Thiacloprid was most potent (EC50 714 µM). In addition, it selectively delayed larval development in wild-type worms at 1 mM.

Conclusions: C. elegans is less susceptible to neonicotinoids than target species of pest insect. We discuss an approach in which this defined low sensitivity may be exploited by heterologous expression of insect nicotinic acetylcholine receptors from both pest and beneficial insects, in transgenic C. elegans with increased cuticle permeability to provide a whole organism assay for species-dependent neonicotinoid effects.

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More information

Accepted/In Press date: 27 February 2017
e-pub ahead of print date: 6 March 2017
Published date: July 2017

Identifiers

Local EPrints ID: 425722
URI: http://eprints.soton.ac.uk/id/eprint/425722
ISSN: 1526-4998
PURE UUID: c33830ae-edbd-4c2a-a469-429aedf351c4
ORCID for Linda Holden-Dye: ORCID iD orcid.org/0000-0002-9704-1217

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Date deposited: 01 Nov 2018 17:30
Last modified: 16 Mar 2024 05:07

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Contributors

Author: Monika M. Kudelska
Author: Vincent O'Connor
Author: Declan Doyle

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