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Identification of a gene involved in the expression of the pathogenicity-related gene XC3814 in Xanthomonas campestris

Identification of a gene involved in the expression of the pathogenicity-related gene XC3814 in Xanthomonas campestris
Identification of a gene involved in the expression of the pathogenicity-related gene XC3814 in Xanthomonas campestris

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of the black rot disease of cruciferous plants. Our previous work had demonstrated that XC3814 is required for full virulence and extracellular polysaccharide production. In this work, the reporter plasmid pL3814sac was constructed by fusing the promoter region of XC3814 to the coding region of the gene sacB, and introduced into Xcc wild-type strain 8004. The resulted strain 8004/pL3814sac was mutagenized randomly by the transposon EZ::Tn5, and 3 mutant strains insensitive to sucrose were isolated. One of the mutants was due to the disruption of the open reading frame XC3882, which was assigned to code a hypothetical protein. To verify whether XC3882 has an impact on the expression level of XC3814, the reporter plasmid pGUS3814 was constructed by fusing the promoter region of XC3814 to the coding region of the gusA gene. This construct was introduced into the wild-type strain 8004 and the XC3882 mutant strain 190A10, which was derived from the transposon Tn5gusA5 insertion. The GUS activity, produced by pGUS3814 in the XC3882 mutant background, was reduced by 81.3% compared to that in the wild type background. These results indicate that the expression of XC3814 is influenced by XC3882.

0253-9772
81-86
Su, Hui Zhao
84d3edd4-cfea-47b0-b741-c6ff2c66b61f
Xiang, Zhi Jiao
b2b9dff2-e773-4ddf-ba50-717a154e4446
Peng, Fang Yin
5ddec937-1dde-4fe7-924b-2d4cb395d423
Li, Rui Fang
1374becb-25e8-4cd2-86de-cc3d82008318
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Lu, Guang Tao
2ff4729d-c048-45fb-9212-a081bd531066
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
Su, Hui Zhao
84d3edd4-cfea-47b0-b741-c6ff2c66b61f
Xiang, Zhi Jiao
b2b9dff2-e773-4ddf-ba50-717a154e4446
Peng, Fang Yin
5ddec937-1dde-4fe7-924b-2d4cb395d423
Li, Rui Fang
1374becb-25e8-4cd2-86de-cc3d82008318
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Lu, Guang Tao
2ff4729d-c048-45fb-9212-a081bd531066
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2

Su, Hui Zhao, Xiang, Zhi Jiao, Peng, Fang Yin, Li, Rui Fang, An, Shi Qi, Lu, Guang Tao and Tang, Ji Liang (2010) Identification of a gene involved in the expression of the pathogenicity-related gene XC3814 in Xanthomonas campestris. Yichuan, 32 (1), 81-86. (doi:10.3724/SP.J.1005.2010.00081).

Record type: Article

Abstract

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of the black rot disease of cruciferous plants. Our previous work had demonstrated that XC3814 is required for full virulence and extracellular polysaccharide production. In this work, the reporter plasmid pL3814sac was constructed by fusing the promoter region of XC3814 to the coding region of the gene sacB, and introduced into Xcc wild-type strain 8004. The resulted strain 8004/pL3814sac was mutagenized randomly by the transposon EZ::Tn5, and 3 mutant strains insensitive to sucrose were isolated. One of the mutants was due to the disruption of the open reading frame XC3882, which was assigned to code a hypothetical protein. To verify whether XC3882 has an impact on the expression level of XC3814, the reporter plasmid pGUS3814 was constructed by fusing the promoter region of XC3814 to the coding region of the gusA gene. This construct was introduced into the wild-type strain 8004 and the XC3882 mutant strain 190A10, which was derived from the transposon Tn5gusA5 insertion. The GUS activity, produced by pGUS3814 in the XC3882 mutant background, was reduced by 81.3% compared to that in the wild type background. These results indicate that the expression of XC3814 is influenced by XC3882.

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Published date: January 2010

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Local EPrints ID: 425811
URI: http://eprints.soton.ac.uk/id/eprint/425811
ISSN: 0253-9772
PURE UUID: 9e22cd72-e352-4862-a20c-3f4db5627bff

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Date deposited: 05 Nov 2018 17:30
Last modified: 05 Jun 2024 19:09

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Contributors

Author: Hui Zhao Su
Author: Zhi Jiao Xiang
Author: Fang Yin Peng
Author: Rui Fang Li
Author: Shi Qi An
Author: Guang Tao Lu
Author: Ji Liang Tang

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