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Systematic mutagenesis of all predicted gntR genes in xanthomonas campestris pv. campestris reveals a GntR family transcriptional regulator controlling hypersensitive response and virulence

Systematic mutagenesis of all predicted gntR genes in xanthomonas campestris pv. campestris reveals a GntR family transcriptional regulator controlling hypersensitive response and virulence
Systematic mutagenesis of all predicted gntR genes in xanthomonas campestris pv. campestris reveals a GntR family transcriptional regulator controlling hypersensitive response and virulence

The GntR family is one of the most abundant and widely distributed groups of helix-turn-helix transcriptional regulators in bacteria. Six open reading frames in the genome of the plant pathogen Xanthomonas campestris pv. campestris were predicted to encode GntR regulators. All six of the predicted GntR-encoding genes were individually mutagenized and mutants from five of them were successfully obtained. Plant disease response assays revealed that one, whose product belongs to the YtrA subfamily and has been named HpaR1, is involved in the hypersensitive response (HR) and virulence. Electrophoretic mobility shift assays and in vitro transcription assays revealed that HpaR1 could repress its own transcription level through binding to its promoter sequence, indicating an autoregulatory feedback inhibition mechanism for HpaR1 expression. Promoter-gusA reporter and reverse-transcription polymerase chain reaction analyses revealed that HpaR1 positively and negatively affects the expression of HR and pathogenicity (hrp) genes in host plant and standard media, respectively. Constitutive expression of the key hrp regulator, hrpG, in the hpaR1 mutant could bypass the requirement of HpaR1 for the induction of wild-type HR, suggesting that HpaR1 regulates the expression of hrp genes that encode the type III secretion system via hrpG.

0894-0282
1027-1039
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Lu, Guang Tao
2ff4729d-c048-45fb-9212-a081bd531066
Su, Hui Zhao
84d3edd4-cfea-47b0-b741-c6ff2c66b61f
Li, Rui Fang
1374becb-25e8-4cd2-86de-cc3d82008318
He, Yong Qiang
054fa877-72e1-456d-85b2-fc607eba4700
Jiang, Bo Le
396adce8-ef31-486e-a44f-e11e246c1b1a
Tang, Dong Jie
46609bbf-2dd4-4ec8-8a7b-a6d6a3b8983b
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Lu, Guang Tao
2ff4729d-c048-45fb-9212-a081bd531066
Su, Hui Zhao
84d3edd4-cfea-47b0-b741-c6ff2c66b61f
Li, Rui Fang
1374becb-25e8-4cd2-86de-cc3d82008318
He, Yong Qiang
054fa877-72e1-456d-85b2-fc607eba4700
Jiang, Bo Le
396adce8-ef31-486e-a44f-e11e246c1b1a
Tang, Dong Jie
46609bbf-2dd4-4ec8-8a7b-a6d6a3b8983b
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2

An, Shi Qi, Lu, Guang Tao, Su, Hui Zhao, Li, Rui Fang, He, Yong Qiang, Jiang, Bo Le, Tang, Dong Jie and Tang, Ji Liang (2011) Systematic mutagenesis of all predicted gntR genes in xanthomonas campestris pv. campestris reveals a GntR family transcriptional regulator controlling hypersensitive response and virulence. Molecular Plant-Microbe Interactions, 24 (9), 1027-1039. (doi:10.1094/MPMI-08-10-0180).

Record type: Article

Abstract

The GntR family is one of the most abundant and widely distributed groups of helix-turn-helix transcriptional regulators in bacteria. Six open reading frames in the genome of the plant pathogen Xanthomonas campestris pv. campestris were predicted to encode GntR regulators. All six of the predicted GntR-encoding genes were individually mutagenized and mutants from five of them were successfully obtained. Plant disease response assays revealed that one, whose product belongs to the YtrA subfamily and has been named HpaR1, is involved in the hypersensitive response (HR) and virulence. Electrophoretic mobility shift assays and in vitro transcription assays revealed that HpaR1 could repress its own transcription level through binding to its promoter sequence, indicating an autoregulatory feedback inhibition mechanism for HpaR1 expression. Promoter-gusA reporter and reverse-transcription polymerase chain reaction analyses revealed that HpaR1 positively and negatively affects the expression of HR and pathogenicity (hrp) genes in host plant and standard media, respectively. Constitutive expression of the key hrp regulator, hrpG, in the hpaR1 mutant could bypass the requirement of HpaR1 for the induction of wild-type HR, suggesting that HpaR1 regulates the expression of hrp genes that encode the type III secretion system via hrpG.

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Published date: September 2011

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Local EPrints ID: 425812
URI: https://eprints.soton.ac.uk/id/eprint/425812
ISSN: 0894-0282
PURE UUID: 2e302bfc-a5d6-4709-af12-2a64c8331ea5

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Date deposited: 05 Nov 2018 17:30
Last modified: 05 Nov 2018 17:30

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Contributors

Author: Shi Qi An
Author: Guang Tao Lu
Author: Hui Zhao Su
Author: Rui Fang Li
Author: Yong Qiang He
Author: Bo Le Jiang
Author: Dong Jie Tang
Author: Ji Liang Tang

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