RsmA regulates biofilm formation in Xanthomonas campestris through a regulatory network involving cyclic di-GMP and the Clp transcription factor
RsmA regulates biofilm formation in Xanthomonas campestris through a regulatory network involving cyclic di-GMP and the Clp transcription factor
Biofilm formation and dispersal in the black rot pathogen Xanthomonas campestris pathovar campestris (Xcc) is influenced by a number of factors. The extracellular mannanase ManA has been implicated in biofilm dispersal whereas biofilm formation requires a putative glycosyl transferase encoded by the xag gene cluster. Previously we demonstrated that the post-transcriptional regulator RsmA exerts a negative regulatory influence on biofilm formation in Xcc. Here we address the mechanisms by which RsmA exerts this action. We show that RsmA binds to the transcripts of three genes encoding GGDEF domain diguanylate cyclases to influence their expression. Accordingly, mutation of rsmA leads to an increase in cellular levels of cyclic di-GMP. This effect is associated with a down-regulation of transcription of manA, but an upregulation of xag gene transcription. Mutation of clp, which encodes a cyclic di-GMP-responsive transcriptional regulator of the CRP-FNR family, has similar divergent effects on the expression of manA and xag. Nevertheless Clp binding to manA and xag promoters is inhibited by cyclic di-GMP. The data support the contention that, in common with other CRP-FNR family members, Clp can act as both an activator and repressor of transcription of different genes to influence biofilm formation as a response to cyclic di-GMP.
Lu, Xiu Hong
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An, Shi Qi
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Tang, Dong Jie
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McCarthy, Yvonne
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Tang, Ji Liang
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Dow, John Maxwell
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Ryan, Robert P.
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21 December 2012
Lu, Xiu Hong
5e0f23b3-815d-4ddd-b0e4-da8db3f10f83
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Dong Jie
46609bbf-2dd4-4ec8-8a7b-a6d6a3b8983b
McCarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
Dow, John Maxwell
a904f493-80b4-4868-999f-af843fff1063
Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298
Lu, Xiu Hong, An, Shi Qi, Tang, Dong Jie, McCarthy, Yvonne, Tang, Ji Liang, Dow, John Maxwell and Ryan, Robert P.
(2012)
RsmA regulates biofilm formation in Xanthomonas campestris through a regulatory network involving cyclic di-GMP and the Clp transcription factor.
PLoS ONE, 7 (12), [e52646].
(doi:10.1371/journal.pone.0052646).
Abstract
Biofilm formation and dispersal in the black rot pathogen Xanthomonas campestris pathovar campestris (Xcc) is influenced by a number of factors. The extracellular mannanase ManA has been implicated in biofilm dispersal whereas biofilm formation requires a putative glycosyl transferase encoded by the xag gene cluster. Previously we demonstrated that the post-transcriptional regulator RsmA exerts a negative regulatory influence on biofilm formation in Xcc. Here we address the mechanisms by which RsmA exerts this action. We show that RsmA binds to the transcripts of three genes encoding GGDEF domain diguanylate cyclases to influence their expression. Accordingly, mutation of rsmA leads to an increase in cellular levels of cyclic di-GMP. This effect is associated with a down-regulation of transcription of manA, but an upregulation of xag gene transcription. Mutation of clp, which encodes a cyclic di-GMP-responsive transcriptional regulator of the CRP-FNR family, has similar divergent effects on the expression of manA and xag. Nevertheless Clp binding to manA and xag promoters is inhibited by cyclic di-GMP. The data support the contention that, in common with other CRP-FNR family members, Clp can act as both an activator and repressor of transcription of different genes to influence biofilm formation as a response to cyclic di-GMP.
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journal.pone.0052646
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Accepted/In Press date: 19 November 2012
Published date: 21 December 2012
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Local EPrints ID: 425813
URI: http://eprints.soton.ac.uk/id/eprint/425813
ISSN: 1932-6203
PURE UUID: d818be2b-901e-4cde-8008-a534e2602b5b
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Date deposited: 05 Nov 2018 17:30
Last modified: 15 Mar 2024 22:29
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Author:
Xiu Hong Lu
Author:
Shi Qi An
Author:
Dong Jie Tang
Author:
Yvonne McCarthy
Author:
Ji Liang Tang
Author:
John Maxwell Dow
Author:
Robert P. Ryan
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